DNA序列分析与PCR扩增技术研究.pdfVIP

前向：

CACCATCATCTAACAGGTCATCAGGTGTCTCAGAACAAACCTGAGCATGACTACTTCCCATAGGCTGTTCTAA

GTTATCTGAAATCAATGGAGAGAAATCTTTAACAGTCTGAACTACTTCTTCATATTCTTGCTTTTTTATTTC

AGGCTTACAATTACTTCCAGGAAGACTTTGTTTATAGACCTCAGGTTGCAAAACCCCTAATCTAAGCATAGCAT

TCAATTTTGGCCCTCTGTTTCTACCTAGTTCTGCTTGAATGTTTTCATCACTGGAACC

反向：

GGTTCCAGTGAAAACATTCAAGCAGAACTAGGAAACAGAGGGCCAAAATTGAATGCTATGCTTATA

GGGGTTTTGCAACCTGAGGTCTATAAACAAAGTCTTCCTGGAAATTAGCATCCTGAAATAAAAAAGCAA

GAATATGAAGAAGTTCAGACTGTTAATACATTCTCTCCATATCTTTCAAACTTAGAACAGCCTAT

GGGAAGTCATGCATCTCAGGTTTGTTCTGAGACACCTGACCTGTTAGGTG

3:read1read2Tn5标记，p5p7PCR4:read1标记+read2标记，p5p7PCR3‑A1

CACCATCATCTAACAGGTCATCAGGTGTCTCAGAACAAACCTGAGCATGACTACTTCCCATAGGCTG

TTCTAAGTTATCTGAAATCAATGGAGAGAAATCTTTAACAGTCTGAACTACTTCTTCATATTCTT

G

C

T

T

T

TTTATTTCAGGCTTACAATTACTTCCAGGAAGACTTTGTCTGTCTCTTATACACATCTCTGAGCGGGCTGGCAAGGCAGACCCTCTGCCGTCTTCTGCTTG

3-A2

CACCATCATCTAACAGGTCATCAGGTGTCTCAGAACAAACCTGAGCATGACTACTTCCCATAGGCTGTTCTAA

GTTATCTGAAATCAATGGAGAGAAATCTTTAACAGTCTGAACTACTTCTTCATATTCTTGCTTTTTTATTTC

AGGCTTACAATTACTTCCAGGAAGACTTTGTTTATAGACCCTGTCTCTTATACACATCTCTGAGCGGGCTGGCA

AGGCAGACCCTCTGCCGTCTTCTGCTTG

3-A4

GCCTCCCTCGCGCCATCAGAGTAAGAGACAGGTTCTAAGTTATCTGAAATCAATGGAGAGAAATCT

TTAACAGTCTGAACTACTTCTTCATATTCTTGCTTTTTTATTTCAGGCTTACAATTACTTCCAGGACTGTCTC

TTATACACATCTCTGAGCGGGCTGGCAAGGCAGACCCTCTGCCGTCTTCTGCTTG

3-A5

GCCTCCCTCGCGCCATCAGAGTTAAGAGACAGACTTAGAACAGCCTATGGGAAGTCATGCATCTCA

GGCTGTCTCTTATACACATCTCTGAGCGGGCTGGCAAGGCAGACCCTCGTATGCCGTCTTCTGCTTG

3-B6

GCCTTGCCAGCCCGCTCAGAGTTAAGAGACAGTTAACAGTCTGAACTACTTCTTCATATTCTTGCTTT

TTTATTTCAGGCTTACAATTACTTCCAGGAAGACTTTGTTTATAGACCTCAGGTTGCAAAACCCCTAATCTAAG

CATAGCATTCAATTTTGGCCCTCTGTTTCTACCTAGTTCTGCTTGAATGTTTTCATCACTGGAACC

3-C4

GCCTTGCCAGCCCGCTCAGAGTTAAGAGACAGGAACTAGGAAACAGAGGGCCAAAATTGAATGCT

ATGCTTATAGGG