医学论文：蟾酥注射液对h22腹水型肝癌的抑制作用及对荷瘤小鼠生存周期的影响.docVIP

【摘要】? 目的 探讨蟾酥注射液对h22腹水型肝癌小鼠肿瘤抑制作用的作用机理。方法 将60只昆明种小鼠随机分为3组。蟾酥注射液组(治疗1组)：每次注射蟾酥注射液1.5 ml/只；环磷酰胺组(治疗2组)：注射环磷酰胺0.5 mg/只(用生理盐水组稀释)；生理盐水组(对照组)：注射生理盐水0.5 ml/只。每组20只。各组均隔5 d注射1次,共给药3次。每组中取10只最后1次注射后5 d,采集血液,检测肿瘤坏死因子；另10只记录小鼠存活情况。结果 蟾酥注射液能不同程度延长腹水型肝癌小鼠的生存期,能使肿瘤坏死因子水平明显下降,与对照组比较,p＜0.01,治疗组之间比较,p＜0.05。结论 蟾酥注射液对h22腹水型肝癌小鼠肿瘤有抑制作用,能够延长生存期。 【关键词】? 蟾酥注射液；腹水型肝癌；生存周期；小鼠　　abstract：objective to investigate the mechanism of secretio bufonis injection on the carcinostatic activities in mice bearing intraperitoneal h22 tumor. methods secretio bufonis injection (1.5 ml) was injected into the abdomind cavity of mice once 5 d for 3 times. cyclophosphamide (0.5 mg/0.5 ml) was injected into other groups. ns (0.5 ml) was injected into control groups. each group were 20 mice. tnf and survival time of mice were observed. results mean survival times of mice bearing intraperitoneal h22 tumor were (28.2±5.8)d for secretio bufonis group, (27.9±9.7)d for cyclophosphamide group, (20.7±4.2)d for control group. the content of tnf in mice bearing intraperitoneal h22 tumor were 2.53±0.14 for secretio bufonis group, 3.02±0.14 for cyclophosphamide group, 3.32±0.2 for control group. the survival times of mice bearing intraperitoneal h22 tumor were prolonged in the groups of secretio bufonis (p0.01) and cyclophosphamide (p0.05). the content of tnf were significantly inhibited in the groups of secretio bufonis (p0.01). conclusion secretio bufonis injection can inhibit the tumor and prolong the survival time of mice bearing intraperitoneal h22 tumor. 　　key words：secretio bufonis injection；bearing intraperitoneal h22 tumor；survival time；mice?　　蟾酥为蟾蜍科动物中华大蟾蜍或黑眶蟾蜍的皮肤腺及耳后腺分泌的白色浆液干燥物。味甘辛,性温,有解毒、消肿、止痛作用。可治疗疮、痈疽发背、瘰疬、慢性骨髓炎、咽喉肿痛、小儿疳积、心衰、风虫牙痛等。有关蟾酥抗炎、抗感染以及抗癌的实验与临床研究文献上已见报道1-3。为了进一步探讨蟾酥抗癌的作用机理,我们将蟾酥注射液接种于h22腹水型肝癌瘤株的昆明种小鼠,观察其生存周期、肿瘤坏死因子变化,并设环磷酰胺及生理盐水组对比。现将结果报道如下。　　1? 实验材料　　1.1? 动物　　昆明种小鼠,体重18～22 g,鼠龄80～100 d,雌雄不限,广州中医药大学实验动物中心提供,动物合格证号：0010387,动物实验设施合格证号：0009285。　　1.2? 药物　　蟾酥注射液,安徽凤阳科苑药业有限公司生产；注射用环磷酰胺由江苏恒瑞医药股份有限公司生产；生理盐水