不同形貌的金纳米材料的电化学生长及其SERS基底的应用.docVIP

  不同形貌的金纳米材料的电化学生长及其 SERS 基底的应用# 刘方菁1,2，钱卫平1,2** 5 10 15 （1. 生物电子学国家重点实验室，南京 210096； 2. 东南大学生物科学与医学工程学院，南京 210096） 摘要：表面增强拉曼散射（SERS）作为一种光学检测方法具有特异性好和灵敏度高的优点， 金、银等金属纳米结构可产生 SERS 效应，应用于化学分子的痕量检测。文章工作利用电化 学方法分两阶段调控纳米结构的生长：第一步调控纳米粒子在铟锡氧化物（ITO）玻璃上成 核，第二步调控在成核位点上纳米结构的原位生长，可控地制备出不同形貌、尺寸以及不同 间距的纳米结构基底。利用尼罗蓝 A（NBA）信号分子对不同基底的 SERS 活性检测，可比 较其形貌、尺寸和阵列结构对 SERS 活性的影响。结果表明，纳米材料基底的 SERS 活性不 但取决于高强度“热点”的产生，还与单位面积内“热点”的密集程度密切相关。本工作所使用 的电化学制备方法无需模板、表面活性剂以及镀膜等材料，具有操作简单、无副产物及其他 损耗、结构可调、低耗环保、设备简单、价格低廉、SERS 增强效果好等优点。在 SERS 基 底定向大规模制备以及痕量检测方面具有应用价值。 关键词：生物医学工程；电化学沉积；表面增强拉曼散射；SERS；花形纳米结构；多形貌 纳米结构 中图分类号：Q63 20 Electrochemical Seeded Growth of Multiple Shapes of Gold Nanostructures and Their Application as SERS Substrates Liu Fangjing1,2, Qian Weiping1,2 (1. State Key Laboratory of Bioelectronics, Southeast University, NanJing 210096; 25 30 35 40 2. School of Biological Science and Medical Engineering, Southeast University, NanJing 210096) Abstract: Surface-enhanced Raman scattering (SERS) is an optical detection method which has the advantages of high specificity and sensitivity. Gold nanostructures are always used to trace detection of chemical molecules because they can produce SERS effect and they are Biocompatible. Our researches used electrochemical and seeded methods to control the growth of nanostructures in two steps. The first step was the nucleation and seed formation of gold on the indium tin oxide (ITO) surface. The second step is the situ growth of gold nanostructures based on seeds. Regulating related parameters of two steps respectively, gold nanostructures with multiple shapes could be synthesized controllably. We used Nile blue A (NBA) as signal molecules to detect the SERS-activity of these gold nanostructures with multiple shapes respectively. The results showed that the gold nanostructures were stable during six months, well reproducible and the SERS-activity of nanostructures depended on the intensity and density of hot spots. The gold nanoparticles array contain