一个携带簇毛麦籽粒硬度基因Dina_Dinb小片段易位系的创制.docVIP

  一个携带簇毛麦籽粒硬度基因 Dina/Dinb 小 片段易位系的创制# 张瑞奇* 5 10 15 20 25 30 35 40 45 （南京农业大学细胞遗传所，作物遗传与种质创新国家重点实验室，南京 210095） 摘要：携带外源优异基因的小片段易位系是重要的种质资源。本研究为了创制携带簇毛麦籽 粒硬度基因 Dina/Dinb 的小片段易位系，以普通小麦-簇毛麦 5VS.5DL 整臂易位系为材料， 通过 60CO-γ 辐射其雌配子体，并以硬质小麦郑麦 9405 为回交亲本，通过 GISH 鉴定，在 BC2F2 后代中获得了一个小片段纯合易位系 NAU5VS-5-5。利用 5VS 特异分子标记、籽粒 硬度相关基因特异分子标记及 C-分带技术综合鉴定结果表明，NAU5VS-5-5 携带簇毛麦 Dina/Dinb 基因，是 5VS-6AS·6AL 小片段顶端纯合易位系。在 BC3F2 群体中，具有纯合易 位染色体的单株与不含易位染色体的单株分离之比符合 1:1，表明易位染色体能够正常传递， 且所有纯合易位染色体的单株均为软质胚乳，而所有不含易位染色体的单株均为硬质胚乳， 表明易位染色体携带的簇毛麦 Dina/Dinb 基因能够显著降低籽粒硬度值。本研究所创制的小 片段易位系及开发的特异分子标记为弱筋小麦品质改良提供了可用的遗传资源。 关键词：小麦；簇毛麦；小片段易位系 中图分类号：S326 Development of a small alien-segment translocation line carrying the softness genes of Haynaldia villosa Zhang Ruiqi (National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, NanJing 210095) Abstract: The wheat-alien small segment translocation (SAST) lines carrying the beneficial genes from wild species are useful genetic stocks for wheat improvement. In this study, in order to introduce the grain hardness-related genes of H. villosa into common wheat, the mature female gametes of whole-arm wheat - H. villosa translocation line T5VS·5DL was 60CO-γ ray irradiated to develop of SAST lines involving 5VS. Among the BC2F2 population, a homozygous SAST line NAU5VS-5-5 was identified by GISH, and the exact fragment sizes were further defined using four 5VS-specific markers and four Ha gene-based markers. The results showed that NAU5VS-5-5 carried the softness-gene Dina/Dinb of H. villosa, and it was identified to be a 5VS-6AS·6AL terminal translocation. The translocation chromosome 5VS-6AS·6AL was proved to be stably inherited to the successive generations. In the BC3F2 generation, the individuals having the homozygous 5VS-6AS·6AL translocation chromosomes all showed soft grain texture, with an approximately 50 percent reduction in the SKCS hardness index compared to that of their backcrossing parent. Both t