L-肉碱对母猪胎盘绒毛膜IGFs和GLUT-1表达水平的影响.docVIP

  L-肉碱对母猪胎盘绒毛膜 IGFs 和 GLUT-1 表达水平的影响# 张玉山，管武太，黄璇，吕艳涛，廖志超，张银芝** 5 （华南农业大学动物科学学院，广州 510642） 摘要：本文旨在研究妊娠期日粮中添加 L-肉碱对母猪胎盘绒毛膜相关基因和蛋白表达的影 响。选择遗传一致、胎次 3-5 胎的长大二元母猪 40 头，随机分为对照组（0mg/kg L-肉碱） 和试验组（50mg/kg L-肉碱）两个处理组，每组 20 头母猪，于母猪自然分娩排出胎盘时， 10 15 20 25 30 35 40 45 采集胎盘绒毛膜样品，通过荧光定量 PCR 和 Western blot 免疫印迹法研究妊娠期日粮中添加 L-肉碱对母猪胎盘绒毛膜相关基因和蛋白表达的影响。结果表明， L-肉碱显著上调了母猪 胎盘绒毛膜中 IGF-1 mRNA 的表达水平（P＜0.05），GLUT-1mRNA 表达量虽没有显著增 加，但也有较明显的上调趋势（P＜0.1），并一定程度上调了 IGF-2 和 IGFBP-3 两个基因的 mRNA 表达量（P＞0.05）；Western blot 免疫印迹结果也表明，L-肉碱显著上调了母猪胎盘 绒毛膜 IGF-1 的蛋白表达量（P＜0.05），极显著上调了 IGF-2 的蛋白表达量（P＜0.01）， GLUT-1 蛋白表达量也有较明显的上调趋势（P＜0.1）。由此可知，日粮中添加 L-肉碱提高 了胎盘绒毛膜中 GLUT-1 和 IGF 系统的 mRNA 和蛋白表达水平，促进了胎盘发育，增强了 胎盘养分转运功能，从而提高了母猪的繁殖性能。 关键词：L-肉碱；母猪；胎盘绒毛膜；IGF-1； IGF-2 ；GLUT-1 中图分类号：S816.79 Effects of Dietary Supplementation of L-carnitine on the IGFs and GLUT-1 Expression Levels of Placental Chorions in Sows ZHANG Yushan, GUAN Wutai, HUANG Xuan, LV Yantao, LIAO Zhichao, ZHANG Yinzhi (College of Animal Science, South Agricultural University Guangzhou 510642) Abstract: The research was conducted to investigate the effects of dietary supplementation of L-carnitine during pregnancy on the IGFs and GLUT-1 expression of placental chorions in sows. A trial with 40 crossbred sows (Yorkshire ×Landrace) was conducted which were assigned to two groups with consideration of their parity number (parity 3 to 5), fed either control diets with low native L-carnitine concentrations or the same diets supplemented with 50 mg of L-carnitine/kg during pregnancy. Collect placental chorionic samples when they were dischanged from sows. IGFs and GLUT-1gene expression by using Real-time quantitative PCR method and their protein expression by using Western Blot method. Real-time quantitative PCR results showed IGF-1 mRNA expression level of placental chorions in sows fed added L-carnitine was significantly higher than that in sows fed the control diet (P＜0.05). GLUT-1 mRNA expression level of chorions tended to be increased in sows supplemented