miRNA-196b过表达对K562细胞增殖、凋亡及Survivin、Cox-2表达的影响.docVIP

  miRNA-196b 过表达对 K562 细胞增殖、凋 亡及 Survivin、Cox-2 表达的影响# 尹虹1，刘玥1，宋艳斌1，马文丽1，郑文岭1，陈滨2** 5 10 15 20 25 30 35 （1. 南方医科大学基因工程研究所，广东广州 510515； 2. 广州市南方医院创伤骨科，广州市广州大道北 1838 号，510515） 摘要：目的：探讨 miRNA-196b 过表达对 K562 细胞增殖、凋亡及 Cox-2、Survivin 基因表 达的影响。方法：实验分为 miRNA-196b 组、siRNA-ABL 组、伊马替尼(IM)组与 K562 组， 采用 CCK-8 法检测细胞增殖；采用 Annexin V-PE 检测细胞凋亡；采用实时荧光定量 PCR 法检测 Cox-2、Survivin、基因的表达情况。结果：miRNA-196b 过表达可以明显抑制 K562 细胞增殖；miRNA-196b 组细胞凋亡率显著高于 K562 组和 siRNA-ABL 组，但低于伊马替 尼组（P0.05）；miRNA-196b 组中 Survivin 基因显著低表达（P0.05）Cox2 基因中无明显 变化（P0.05）。结论：miRNA-196b 对 K562 细胞的增殖抑制和诱导凋亡有显著作用； miRNA-196b 过表达可下调 Survivin 基因的表达，为 miRNA-196b 作为慢性粒细胞性白血病 的治疗靶点提供了一定依据。 关键词：miRNA-196b；siRNA-ABL；伊马替尼；K562 细胞；细胞增殖；细胞凋亡 中图分类号：R34 Effects of miRNA-196b overexpression on Proliferation, Apoptosis and Survivin,Cox-2 Expression of K562 Cells Yin hong1, Liu Yue1, Song Yanbin1, Ma Wenli1, Zheng Wenling1, Chen Bin2 (1. Institute of Genetic Engineering,Southern Medical University,Guangzhou 510515,China; 2. Department of orthopaedics and Traumatology,Nanfang Hospital,Southern Medical University,1838 North Guangzhou Avenue,Guangzhou 510515,China) Abstract: To investigate the effects of miRNA-196b overexpression on proliferation, apoptosis and Cox-2，survivin genes expression of K562 cells. Methods Four groups including miRNA-196b, siRNA-ABL, Imatinib（IM） and K562 control group were set up in this study. The cell proliferation and apoptosis were measured by CCK-8 assay and Annexin V-FITC/PI, respectively. The expression of Cox-2 and survivin genes at the mRNA level was detected by RT-qPCR. Results The proliferation of K562 cells could be significantly inhibited by miRNA-196b overexpression; Compared of the four groups of apoptosis rate, miRNA-196b group significantly higher than K562 and siRNA-ABL groups, but less than imatinib group (P 0.05). The expression of survivin gene in miRNA-196b was donwregulated(P 0.05),but the expression of cox-2 gene in miRNA-196b group had no significant difference（P0.05）.Conclusion The mi