Notch1信号分子的表达及脑泰方的调节作用.docVIP

  局灶性脑缺血大鼠 VEGF/Notch1 信号分子 的表达及脑泰方的调节作用# 陈懿1，廖君2，朱惠斌3，葛金文4** 5 10 15 20 25 30 35 40 （1. 湖南中医药大学医学院，长沙 410208； 2. 湖南中医药大学基础医学院，长沙 410208； 3. 湖南中医药大学附属第一医院，长沙 410007； 4. 湖南中医药大学科技处，长沙 410208） 摘要：目的：从 VEGF-Notch1 信号通路观察脑泰方对局灶性脑缺血大鼠的治疗性血管新生 样作用。方法：将 65 只 SD 大鼠随机分为假手术组、MCAO 组(模型组)、脑泰方组（NTF）、 NTF+ ED（重组人血管内皮抑制素）组。线栓法制备大鼠大脑中动脉栓塞（MCAO）模型， NTF 组及 NTF+ ED 组分别于造模成功后 24h 灌胃 NTF 或灌胃 NTF 加腹腔注射 ED，术后 1、 7 、14、28d 取脑，采用 RT-PCR、Western blot 方法观察 VEGF、Flk-1、Notch1 的表达。 结果：与 MCAO 组比较，NTF 组大鼠各时间点缺血侧脑组织 VEGF、Flk-1、Notch1 表达水 平升高，在第 14d 时达高峰，而加用血管内皮生成抑制素恩度后，这种增高效应随即消失。 结论：脑泰方可能通过上调 VEGF- Notch1 信号通路的表达，以达到治疗性血管新生样作用。 关键词：中西医结合基础；脑缺血；Notch；VEGF；血管新生；脑泰方 中图分类号：R255.2 Effects of Naotaifang extract on the expression of VEGF/Notch1 signaling pathway in rats with focal cerebral ischemia CHEN Yi1, LIAO Jun2, ZHU Huibin3, GE Jinwen4 (1. College of Medicine, Hunan University of Chinese Traditional Medicine, ChangSha 410208; 2. College of Basic Medicine, Hunan University of Chinese Traditional Medicine, ChangSha 410208; 3. the first affiliated hospital, Hunan University of Chinese Traditional Medicine, ChangSha 410007; 4. Department of Science and Technoloy, Hunan University of Chinese Traditional Medicine, ChangSha 410208) Abstract: Objective The effects of therapeutic angiogenesis of Naotangfang extracts were investigated by VEGF-Notch1 signaling pathway in ischemic brain damage in rats. Methods 65 Sprague-Dawley(SD) rats were randomly divided into 4 groups, namely, sham operation group, cerebral ischemia model group (MCAO groups), Naotaifang extract groups (NTF groups), and NTF+ Endostatin (ED) groups. Cerebral ischemia was established by modified Zea Longa’s method. At 24h post-MCAO, endostatin was injected into abdominal cavity and NTF extract was intragastrically administrated. Rats are sacrificed on days 1, 7, 14 and 28 d post-MCAO. VEGF, Flk-1 and Notch1 protein and mRNA levels in ischemic zones were determined by RT-PCR and western blot. Results: Compared with the model groups, the