纳豆中VB1的UV-VIS分析研究.pdfVIP

纳豆中 VB1 的UV-VIS 分析研究 摘 要 利用紫外分光光度法测定了纳豆中维生素B 含量，分为酸性介质和碱性介质B 1 两种方法。在碱性介质中，测定波长 433nm ，其最佳测试条件为:显色剂用量（对 甲基氨基苯酚硫酸酯）1.0mL,显色时间 15min,碱性介质（浓氨水）用量 1.0mL, 2 其线性回归方程为y=0.50302+0.49659x,相关系数R =0.9926,线性范围 0.2mg/L～ 2.5mg/L,平均回收率为 99.40%, RSD为 0.2%，该方法简单，易操作，重现性好。 在酸性介质中，本实验选定了pH=4 的条件对维生素B 作测量。测定波长 266nmB 1 线性范围：0.2mg/L～2.5mg/L工作曲线方程：y=0.74884+0.74821x ，线性相关系 2 数：R =0.9931 ，回收率 99.55%,精密度 RSD=0.6% 。该方法同样简单，易操作， 重现性好。 关键词：纳豆，维生素B ，紫外分光光度法B 1 I 纳豆中 VB1 的UV-VIS 分析研究 Abstract The ultravio let spectrophoto metric method was used to determinate vitaminB1 in natto.There two condition in test:acid and base.In the base wavelength for determination was 433nm ,the optimum test conditions were as follows:dose of the developr was1.0mL,develop time was 15min,dose of the alkaline medium was1.0mL.The linear regression equation was y=0.50302+0.49659x,with a linear range of 0.2mg/L ～ 2.5mg/L 2 (R=0.9926)and the average recovery rate was99.40%with0.2%of RSD.The results showed that the method was simple and easy to operate with good repeability.In the acid, wavelength for determination was 266nm, the experiment accomplished in the pH=4 NaAC-HAC buffer water system The linear regression equation was y=0.74884+0.74821x,with a linear range of 2 0.2mg/L ～ 2.5mg/L (R =0.9931)and the average recovery rate was99.55%with0.6%of RSD. The results also showed that the method was simple and easy to operate with good repeability. Keywords: natto,vitaminB ,UVspectrophotometr