电针对局灶性脑缺血再灌注大鼠神经行为学障碍的影响.docVIP

  电针对局灶性脑缺血再灌注大鼠神经行为 学障碍的影响# 张晓明，瞿涛，丁玲，左朝，陈熠路，陈邦国** 5 10 15 20 25 30 35 40 （湖北中医药大学针灸骨伤学院） 摘要：目的：观察电针对局灶性脑缺血再灌注大鼠缺血区形态学损伤及行为学障碍的影响。 方法：30 只清洁级雄性 SD 大鼠随机分为假手术组、模型组、电针组，每组 10 只，模型组 与电针组大鼠采用线栓法栓塞大脑中动脉 60 min 后拔出线栓恢复血流，电针组在缺血再灌 注 2h 后开始电针刺激（百会、水沟、足三里，疏密波，频率 2Hz/15Hz）30 min，每隔 12h 重复刺激 1 次。再灌注后 72 h，采用 Zea-Longa 评分方法观察电针对缺血再灌注大鼠神经行 为学障碍的影响，HE 染色观察电针对缺血区病理形态损伤的影响。结果：电针组大鼠脑组 织病理形态学损伤较模型组明显改善；神经行为学障碍明显轻于模型组；结论：电针刺激可 明显减轻脑缺血再灌注大鼠脑组织损伤、对脑缺血再灌注大鼠神经功能有良性调节作用。 关键词：电针；脑缺血再灌注；神经行为学；形态学 中图分类号：R245 Effects of electroacupuncture against neurological deficit of rat with focal cerebral ischemia/reperfusion ZHANG Xiaoming1, QU Tao1, DI ling1, ZUO Zhao2, CHEN Yilu1, CHEN Bangguo1 (1. College of Acupuncture and Orthopedics,Hubei University of Chinese Medicine; 2. College of Acupuncture and Orthopedics, Hubei University of Chinese Medicine) Abstract: I Objective: To observe the effects of electroacupuncture(EA) against focal cerebral ischemia/reperfusion (IR) injury in rats by studying morphology and neurological deficit. Methods: Thirty male SD rats of clean grade were randomly divided into sham operation group, model group and EA group, n = 10, model group and EA group rats were modified the middle cerebral artery occlusion(MCAO) models with nylon monofilament, the filament was left in place for 60 min and then withdrawn to let the blood reperfuse. EA group was stimulated with EA (at the acupoints of Baihui, Shuigou, Zusanli, sparse and dense wave alternately, frequence of 2HZ/15HZ) for 30 min, repeted the stimulation every other 12 h. After 72 h of reperfusion, neurological deficit was measured according to the method of Zea-Longa to observe the effect of EA against neurological deficit after IR, Hematoxylin and Eosin(HE) staining to observe the effect of EA against pathological injury. Results: Pathological injury in brain tissue of the EA group significantly improved than the model group. The neurological difict was significantly lighter than the model group. Conclusion: The stim