茶多酚对大鼠脑缺血-再灌注损伤后细胞凋亡和p38 信号通路的影响.docVIP

中青年优秀论文评奖 茶多酚对大鼠脑缺血再灌注损伤细胞凋亡p38信号通路的 710004 赵红霞 薛荣亮 魏欣 高慧 蔡英敏 【摘要】目的 探讨茶多酚对全脑缺血再灌注大鼠海马CA1区凋亡细胞及p38信号转导通路mg/kg，其余各组则给予腹腔注射等量生理盐水。各组在预定时间点提取脑组织制备病理切片，HE染色观察海马CA1区神经元形态结构改变；免疫组化染色观察磷酸化P38蛋白PO组少量表达，在IR组(Po．05)，而TP组于各时间点凋亡细胞表达介于IR组和PO(Po．05)；与PO组比较，IR组在再灌注后各时点p38蛋白表达显著增强(P0．05)，TP组较PO组表达增强但较IR组表达减弱(Po．05)。结论 茶多酚可抑制大鼠全脑缺血/再灌注时p38蛋白的活性，进而减少海马椎体细胞凋亡，对脑缺血/再灌注损伤具有保护作用。缺血/再灌注损伤； 凋亡； P38蛋白/ Reperfusion injury in rats 【Abstract】 Objective To investigate the impact of Tea Polyphenols on apoptosis and P38MAPK Signaling Pathway after Cerebral Ischemia/ Reperfusion injury in rats. Methods 国家自然科学基金资助项目 108 healthy clean SD rats were randomly divided into 3 groups: Pseudo-operation group(PO group, n=36), ischemia/reperfusion group (IR group, n=36) and tea polyphenols group (TP, n = 36). each group with 6 pionts,and each pionts have 6 rats. Global cerebral I／R was produced by 4-vessel occlusion method. In TP group 200 mg/kg Tea Polyphenols was given when reperfusion was end, at the same time, in PO group and IR group was given normal saline. to extract the brain tissue and to make the Pathology Slice in Schedule. To investigate the form and the structure of neurons in hippocampal CA1 region; Phosphorylated p38 expression was assessed by immuno-histochemistry; to observe the apoptosis by TUNEL staining. Results After Cerebral Ischemia/ Reperfusion injury, the apoptotic cells expressed less in PO group, in IR group it was increased notablely(Po．05),and in TP group , the apoptotic cells expressed between PO group and IR group(Po．05);to Compared with PO group, the express of p38 protein increased notablely in IR group(Po．05), and in TP group , it was expressed between PO group and IR group(Po．05). Conclusionafter Cerebral Ischemia/ Reperfusion injury ,the Tea Polyphenols can restrain the active of the p38 protein, therefor, the apoptotic cells was Reduced, so the Tea Polyphenols can be to protect the nerve cells. 【KEY WORDS】 TP; brain; ischemia/ reperfusion injury