超临界CO2萃取红莓种子有效成分实验条件的探索.pdf

超临界 CO2 萃取红莓种子有效成分实验条件的探索 中文摘要 本文介绍了超临界流体的性质及其萃取原理和流程，归纳了超临界 CO2 萃取的应用 领域，特别是对超临界 CO2 萃取植物有效成分的国内外研究进展进行了综述。 用超临界 CO2 萃取红莓种子中的有效成分，然后用高效液相色谱法直接测定萃取物 中有效成分的含量，从而测得红莓种子中有效成分的含量。超临界流体萃取的条件是： 萃取压力为小于等于 30MPa ，萃取温度为小于等于 50 摄氏度。高效液相色谱测定条件为： 流动相：乙腈和水之比为 9 ∶1；检测波长为 230nm ，应用于植物中有效成分的测定。 关键词：超临界流体萃取 高效液相色谱 红莓种子 Research of availability component of the cranberry seed with supercritical CO extraction’s experiment condition 2 Kong Lichun (Department of Chemical Engineering, Beijing institute of Petrochemical Technology,Beijing,102617) ABSTRACT Characteristics of supercritical fluid and its extraction principle and process were introduced. A review was given on the application of supercritical co2 extraction especially on the extraction of natural products. The general rules of supercritical co2 extraction natural products were also explored. Availability component was extracted from the cranberry seed by using supercritical carbon dioxide fluid extraction (SFE). Its contents in the extract were determined directly by high performance liquid chromatography and the availability component in the cranberry was obtained. The SFE conditions were as follows : Pre-soaker: ethanol, extraction pressure:≤ 30Mpa, extraction temperature: ≤ 50 ℃ . The HPLC conditions were: mobile phase : acetonitrile and water with the ratio of 9 ∶1,detection wavelength :230nm. The method can by used to determine the contents of availability component, pharmaceutical preparations and other plants. Key words: Supercritic