48份分蘖洋葱种质资源遗传多样性的SSR和AFLP标记分析.docVIP

  Evaluation of Genetic Diversity of 48 Potato Onion (Allium cepa var. aggregatum Don.) Germplasms Using SSR and 5 10 15 20 25 AFLP markers# JIN Xue, WU Fengzhi** (Northeast Agricultural University, Mucai 59, Xiangfang, Harbin 150030, P.R. China) Abstract: Simple sequence repeat (SSR) markers and amplified fragment length polymorphism (AFLP) were used to detect the genetic diversity of 48 potato onion germplasms from Heilongjiang and Jilin provinces in China. Twenty three SSR primers with rich polymorphism, stable amplification and good repeatability were screened from one hundred and one SSR primers. With these screened primers, 116 bands were identified, of which 66 bands were polymorphic. The genetic similarity was estimated with the Nei-Li coefficient, and results showed that the genetic similarity coefficients of 48 potato onion germplasms ranged from 0.61 to 0.91. Ten AFLP primers with rich polymorphism, stable amplification and good repeatability were screened from sixty four AFLP primers. With these screened primers, 470 bands were identified, of which 288 bands were polymorphic. The genetic similarity was estimated with the Nei-Li coefficient, and results showed that the genetic similarity coefficients of 48 potato onion germplasms ranged from 0.59 to 0.95. The clustering results of SSR and AFLP markers were basically identical, and both methods showed that the cluster results of potato onion obviously correlated with their geographic origins. Key words: Allium cepa var. aggregatum; Genetic diversity; SSR; AFLP 0 Introduction Potato onion (Allium cepa var. agrogatum Don.), which belongs to the Allium genera of Liliaceae family, is an annual cultivation herbaceous plant. It is an important vegetable worldwide and condiments because of it rich nutrients and high medicinal value, and widely cultivated in the northeast area of China, such as Heilongjiang and Jilin Provinces. Because it has short growth period, not easy to be affected by diseases or pests,