Klotho基因在残余肾模型大鼠肾脏的表达及淫羊藿、黄芪、大黄复方的干预作用研究.docVIP

  Klotho 基因在残余肾模型大鼠肾脏的表达 及淫羊藿、黄芪、大黄复方的干预作用研究 #  5 10 15 20 25 30 35 40  邹新蓉1,2，王小琴1,2，王长江1,2，周慧兰1,2** （1. 湖北中医药大学附属医院，武汉 430061； 2. 湖北省中医院,武汉 430061） 摘要：目的 探讨 klotho 基因在残余肾模型大鼠肾脏的表达变化及淫羊藿、黄芪、大黄复方 对其表达的影响。方法 应用 5/6 肾切除建立残余肾大鼠模型，随机分为正常对照组、假手 术组、模型组、骨化三醇组、中药复方 2.25 g/ml 剂量组、中药复方 4.5 g/ml 剂量组，给予 相应药物灌胃 8 周。给药前、后检测大鼠 BUN、Scr、Ccr、HGB，用药结束取肾组织行病 理检查，Real-time PCR、Western blot 检测 klotho 表达。结果 中药复方 2.25 g/ml、中药复 方 4.5 g/ml 组大鼠 BUN、Scr 与骨化三醇组、模型组比较明显降低；中药复方 2.25 g/ml、中 药复方 4.5 g/ml 组 Ccr、HGB 与模型组比较明显升高；模型组大鼠肾脏病理表现肾小球局灶 节段硬化、小管萎缩塌陷，中药复方干预后病理改变减轻；模型组、骨化三醇组大鼠肾组织 klotho 基因、klotho 蛋白表达显著低于正常组和假手术组，中药复方 2.25 g/ml、中药复方 4.5 g/ml 组 klotho 基因、klotho 蛋白表达明显高于模型组。结论 klotho 基因在残余肾模型大鼠 肾脏的表达降低，淫羊藿、黄芪、大黄复方能上调残余肾组织 klotho 基因表达、减轻病理改 变、减缓肾衰竭进程。 关键词：klotho 基因；残余肾；中药 中图分类号：R259 Researches on Klotho gene’s expression in the kidney of the remnant kidney model rat and the intervention effects of epimedium, astragalus root, rhubarb compound ZOU Xinrong1,2, WANG Xiaoqin1,2, WANG Changjiang1,2, ZHOU Huilan1,2 (1. Affiliated Hospital,Hubei University of Traditional Chinese Medicine,Wuhan 430061; 2. Hubei Provincial Hospital of Traditional Chinese Medicine,Wuhan 430061) Abstract: Objectives To investigate the expressing change of Klotho Gene in the kidney of remnant kidney model rat and the influences of epimedium, astragalus, rhubarb compound on the expression. Methods Establish the remnant kidney rat model by using 5/6nephrectomy. They are randomly divided into normal control group,sham operation group, model group, ossification in three alcohol group, Chinese herbal compound 2.25 g/ml dose group, Chinese herbal compound 4.5 g/ml dose group,and are given relative drugs by gavage for 8 weeks.Check BUN、Scr、Ccr、HGB of these rats before and after giving drugs. Have histopathological examination by taking kidney tissues after giving drugs.Check the expression of klotho by Real-time PCR、 Western blot. Results Compared to ossification in three alcohol group and model group,BUN、Scr of rats in Chinese he