三种蛋白染色方法在SDS聚丙烯酰胺凝胶电泳中的应用.pdfVIP

2000 12 24 4 292 Bull Acad Mil M d Sci, D c 2000; Vol 24 No 4 SDS , , , , ( 军事医学科学院野战输血研究所, 北京 100850) [ ] : 比较SDS聚丙烯酰胺凝胶电泳( SDS- PAGE) 中蛋白染色的3种方法: 将SDS PAGE 中的蛋白分别用银染铜染和考马斯亮 染色, 并对铜染或考马斯亮 染色后的蛋白进行相 应的复染: 快速银染法灵敏度最高, 但操作步骤繁琐, 染色时间约50 min; 考马斯亮 染色灵 敏度最低, 需染色和脱色, 时间约为1 h; 铜染灵敏度介于银染和考马斯亮 染色之间, 但染色时间 仅5 min, 不需脱色, 且可直接用考马斯亮 或快速银染法复染: 用SDSPAGE 进行蛋白检测 时, 铜染可作为蛋白快速分析的首选方法 [ ] 电泳, 聚丙酰凝胶; 蛋白染色方法; 蛋白质类; 银染色 [ ] Q503 [ ] A [ ] 2000) Application of three kinds of staining methods for protein in SDSPAGE CHEN L in , ZHANG Yadong , ZHA O Yanhua, MA p ing , BU Fongrong ( Institut of Fi ld Transfusion, Acad my of Military M dical Sci nc s, B ijing 100850, China) [Abstract] Objective: To compar thr kinds of staining m thods for prot ins in SDSPAGE. Methods: Th prot ins in SDSPAGEw r stain d with coomassi brilliant blu staining, copp r staining and silv r stain ing. Th n th prot ins w r r stain d aft r coomassi brilliant blu staining or copp r staining. esults:Th silv r staining was th most s nsitiv , th s nsitivity of th copp r staining was th form r b tw n that of silv r staining and th coomassi brilliant blu staining. It took only fiv minut s to stain prot ins with copp r stain ing. Aft r copp r staining, th prot ins could b r stain d by silv r staining or coomassi brilliant blu stain ing. Conclusions: Copp r staining could b th first choic for rapid analysis of prot ins in SDSPAGE. [Key words] l ctrophor sis, polyacrylamid g l; prot in staining; prot ins; silv r staining SDSPAGE Shapiro 1967 , , 1 SDSDTT , 1. 1 , SDS , ( BSA) ( Sigma , ) ; ( S rva , ) ; ( ) ; SDSPAGE SDSPAGE (Ho f r ) 1. 2