改良羊水原位培育法进行产前诊断的研究.pdfVIP

现代妇产科进展 2003 年 7 月第 12 卷第 4 期 　Prog Obstet Gynecol ,July 2003 ,Vol. 12 ,No. 4 ·论著· 改良羊水原位培养法进行产前诊断的研究 许争峰 ,胡娅莉 ,朱瑞芳 (南京大学医学院附属鼓楼医院产前诊断中心 ,南京 　210008) 　　【摘要】　目的 :探讨改良羊水原位培养法在产前诊断中的价值。方法 :抽取 138 例孕 16～30 周孕妇的羊水 ,用全营养培养基 4ml + 羊水细胞混悬液 5ml 接种培养 ,适时改良法 原位收获制片、分析。结果 : 138 例羊水培养均获成功 ,成功率 100 % ;平均培养时间为 7 天 ;平均可分析核型数 38 个 ,发现染色体异常 4 例。结论 :用此法培养羊水成功率高 ,培 养时间短 ,可分析核型多 ,培养适用范围宽 ,可满足临床产前诊断的要求。 【关键词】　羊水 ;产前诊断 ;染色体 ( ) 中图分类号:R714. 15 　文献标识码 :A 　文章编号 :1004 - 7379 2003 04 - 0273 - 03 A study on the improved amniotic fluid cells culture in situ for prenatal diagnosis. Xu Zhengfeng , Hu Yali , Zhu Ruifang . Centre of Prenatal Diagnosis , the Affiliated Hospital of Nanjing University Medical College , Nanjing 210008 【Abstract 】　Objective :To explore the value to apply improved amniotic fluid cells culture method in situ for prenatal diagnosis. Methods :Amniotic fluid ,which was extracted from pregnant women during 16～30th weeks gestation ( n = 138) was inoculated and cultured by all nutrition cul ture medium (4ml) and amniotic fluid cells mixed liquid (5ml) ,then they were gathered to set up chromosome according to improved method in proper time. Results :138 amniotic fluid cells culture all gained success ,the success rate was 100 % ;the average culture time was 7 days ;the number of average analysed karyotype was 38 ;abnormal karyotype that had been found was 4. Conclusion :Am niotic fluid cells culture according to improved method in situ has higher success rate ,shorter culture time ,better karyotype ,wider applied range than that of routine amniocentesis ,so it ensure clinic ap plication for prenatal diagnosis. 【Key words 】　Amniotic fluid ;Prenatal diagnosis ;Chromosomes 　　1966 年 Steele 和 Breg 1 首次培养羊水细胞成功