人参生长发育过程中茎内5种酶活性的初步研究.pdfVIP

吉林农业大学学报 2011，09 Journal of Jilin Agricultural University E-mail:jlndxb@ 人参生长发育过程中茎内 5 种酶活性的初步研究* ** 张 惠，赵 雨 ，王思明，刘美辰，李晓华 长春中医药大学研发中心，长春 130117 摘 要：以五年生人参茎为供试材料，分别于人参展叶期、开花期、结果期、果后参根生长期、枯萎期采集人参茎样 品，采用中性缓冲液制备粗酶液，应用分光光度法测定硝酸还原酶（NR ）、超氧化物歧化酶（SOD）、谷丙转氨酶（GPT ）、 葡萄糖磷酸异构酶（GPI ）、延胡索酸酶（FUM ）5 种酶的活力。结果表明：人参生长发育过程中，茎内 NR 和 FUM 均在展叶期和开花期出现活力高峰，SOD 、GPT 、GPI 的活力在结果期较高。5 种酶的活性变化特点可以为人参的种 植优选提供理论依据。 关键词：人参茎；硝酸还原酶；超氧化物歧化酶；谷丙转氨酶；葡萄糖磷酸异构酶；延胡索酸酶 中图分类号：R284.1 ；S567.51 文献标识码：A 文章编号:1000-5684(2011) DOI: CNKI:22-1100/S0928.001 /kcms/detail/22.1100.S0928.001.html 网络出版地址： Preliminary Study on Activity of Five Enzymes in Ginseng Stems during Growth and Development ZHANG Hui, ZHAO Yu, WANG Si-ming, LIU Mei-chen, LI Xiao-hua Research and Development Center, Changchun University of Traditional Chinese Medicine, Changchun 130117, China Abstract: Using five-year old ginseng stems as material, changes of nitrae reductase (NR), superoxide dismutase (SOD), glutamic-pyruvic transaminase (GPT), glucose phosphate isomerase (GPI) and fumarase(FUM) activity of ginseng stems during growth and development were studied. Ginseng stem samples were collected respectively at the spreading stage, the flowering stage, fruit setting stage, the root growing after fruit stage and the withering stage. Neutral buffer solution was adopted to extract the enzyme solution of radix ginseng. Spectrophotometry was used to test the activities of NR, SOD, GPT, GPI and FUM. The results showed that in the growth and development process of ginseng stems, the activity of NR and FUM have the praks of the activity during the speading stage and the flowering stage. The activity of SOD, GPT and GPI during the fruit setting stage