EGFR酪氨酸激酶抑制剂对体外培养Tca8113细胞的作用.docVIP

  EGFR 酪氨酸激酶抑制剂对体外培养 Tca8113 细胞的作用 李志强，杨兰，雷飞，王飞** 5 10 15 20 25 30 35 40 45 （兰州大学第二医院口腔颌面外科，兰州 730000） 摘要：目的 了解表皮生长因子受体（epidermal growth factor receptor，EGFR）在舌鳞癌组 织中的表达以及表皮生长因子受体酪氨酸激酶抑制剂对于体外培养舌癌细胞的作用。方法 采用免疫组织化学方法检测舌鳞癌组织及人舌鳞癌 Tca8113 细胞中的表皮生长因子受体的 表达；采用不同浓度的表皮生长因子受体酪氨酸激酶抑制剂处理体外培养人舌鳞癌 Tca8113 细胞 24~72 小时，MTT 检测细胞增殖活性；采用流式细胞术观察细胞凋亡情况。结果 43 例舌鳞癌组织中 EGFR 阳性表达 31 例，阴性表达 12 例。其阳性表达率 72.09%，人舌鳞癌 Tca8113 细胞中 EGFR 为阳性表达。应用表皮生长因子酪氨酸激酶抑制剂处理人舌癌 Tca8113 细胞后，细胞增殖活性降低，细胞凋亡增加，其效果受药物作用浓度及作用时间的影响。结 论 EGFR 在舌鳞癌组织中有较高程度的表达，表皮生长因子受体酪氨酸激酶抑制剂对于人 舌癌 Tca8113 细胞的增殖具有抑制作用。 关键词：舌鳞癌；表皮生长因子受体；表皮生长因子酪氨酸激酶抑制剂；细胞凋亡 中图分类号：R780.2 Effects of epidermal growth factor receptor tyrosine kinase inhibitor on Tca8113 in vitro Li Zhiqiang, Yang Lan, Lei Fei, Wang Fei (Lanzhou university second hospital oral and maxillofacial surgery, LanZhou 730000) Abstract: Objective: To investigate the expression of epidermal growth factor receptor in human tongue squamous carcinoma and the effects of epidermal growth factor receptor tyrosine kinase inhibitor on Tca8113 cell in vitro. Methods: Using SP immunohistochemical analysis to detect the expression of epidermal growth factor receptor in human tongue squamous carcinoma and the Tca8113 cell; Using different concentration of epidermal growth factor receptor tyrosine kinase inhibitor treat the Tca8113 cell in vitro 24 to 72 hours, MTT method to detect cell proliferation activity, Flow cytometry to observe apoptosis situation. Result: 31cases of EGFR positive expression an 12 cases of EGFR negative expression in 43 patients with tongue squamous carcinoma.Tca8113 cell tested positive for EGFR expression. Application of epidermal growth factor tyrosine kinase inhibitor treat Tca8113 cell, cell proliferation activity decreased and apoptosis increases; the effect of drug action by the influence of the concentration and duration. Conclusion: EGFR in human tongue squamous carcinoma tissues have a higher degree of expression, Epidermal growth factor receptor tyrosine kina