CdSe_ZnS量子点对雄性尼罗罗非鱼红细胞微核率及核异常率的影响.doc

  CdSe/ZnS 量子点对雄性尼罗罗非鱼红细胞 微核率及核异常率的影响# 江星，张耀光，金星星，陈幕飞，王川，金丽** 5 10 15 20 25 30 35 40 （西南大学生命科学学院，淡水鱼类资源与生殖发育教育部重点实验室，重庆 400715） 摘要：为研究 CdSe/ZnS 量子点对尼罗罗非鱼的遗传毒性，分别设置三个对照组，分别是空 白对照组、NaCl 对照组和 Cd2+对照组，以及 4 个量子点处理组，浓度分别为 20nmol/L、 200mol/L、2000mol/L 和 4000nmol/L，以腹腔注射的方式对雄性尼罗罗非鱼体(Oreochromis niloticus）进行暴露，暴露后 1d、4d、7d、14d 及 28d 进行静脉取血制作血涂片，通过微核 率、核异常率及总核异常率检测其遗传毒性。结果显示，各 NaCl 对照组与空白对照组均没 有显著性差异。暴露 1d 后各量子点处理组产生的微核率达到峰值后总体呈下降趋势。Cd2+ 对照组的微核率在整个实验期间均与 NaCl 对照组有显著性差异，其微核率 14d 达到峰值。 核质外凸和核质内凹是核异常的主要类型，核固缩及不均等缢裂细胞类型在前期易被诱导。 结果表明，量子点外壳及表面修饰基团的综合作用对鱼体有诱导微核率及核异常率增加的效 应，但随着时间的推移，罗非鱼能逐步解除该负面效应。由此推测，鱼体最终能解除由 QDs 诱导的遗传毒性。 关键词：量子点；尼罗罗非鱼；微核；核异常；遗传毒性 中图分类号：Q461 THE EFFECT OF CdSe/ZnS QDs INDUCES ERYTHROCYTE MICROMUCLEUS AND NUCLEAR ANOMALIES IN MATE Oreochromis niloticus JIANG Xiang, ZHANG Yaoguang, JIN Xingxing, CHEN Mufei, WANG Chuan, JIN Li (Ley Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, School of Life Sience, Southwest University,ChongQing 400715) Abstract: In order to assess the potential genotoxicity of CdSe/ZnS QDs on mate Oreochromis niloticus, this research sets three control are that blank control, NaCl control and Cd2+concrol ,four QDs treatment are that 20nmol/L, 200mol/L, 2000mol/L and 4000nmol/LQDs concentrations treatment, respectively, the fishes were exposured to active substance by intraperitoneal infection, fishes were analyzed on 1d, 4d, 7d, 14d and 28d post treatment by making blood smears and testing the frequency of micronucleus, frequency of nuclear abnormality and total frequency of nuclear abnormality. The results showed that the frequency of micronucleus reach the maximum after QDs exposure 1 d, after that the trend decline. One-way ANOVA analysis between each blank control group and NaCl control group showed no significant difference during the whole experiment. The micronucleus of Cd2+ control group reach the maximum on 14d post treatment, One-way ANOVA analysis between each Cd2+ control group and NaCl control gr