苯巴比妥对新生大鼠缺氧缺血性脑损伤的保护及对脑组织中KCC2的影响.docVIP

  苯巴比妥对新生大鼠缺氧缺血性脑损伤的 保护及对脑组织中 KCC2 的影响# 王淙1，张素培1，马俊远1，李强2,3，李杰2,3* 5 10 15 20 25 30 35 40 45 （1. 郑州大学药学院，郑州 450001； 2. 河南中医学院第一附属医院，郑州 450000； 3. 河南省病毒性疾病中医药防治重点实验室，郑州 450000） 摘要：目的： 观察苯巴比妥对新生大鼠缺氧缺血性脑损伤（HIBD）的保护及对脑组织中 KCC2 的影响，探讨苯巴比妥的脑损伤保护作用机制。方法： 7 日龄 Sprague-Dawley (SD) 大鼠，随机分成三组：假手术组、HIBD 模型组、苯巴比妥组。HIBD 模型组和苯巴比妥组 参照 Rice-Vannucci 方法制作新生大鼠 HIBD 模型, 假手术组仅游离左侧颈总动脉，不作缺 氧缺血处理。给药完成后，一部分动物处死,取脑组织切片进行 TTC 染色, 其余部分动物饲 养 4 周龄时用水迷宫实验检测其学习和记忆能力。此外，免疫蛋白印迹法检测缺血侧脑组织 KCC2( 钾氯共转运体-2)蛋白的表达。结果： 与 HIBD 模型组相比，苯巴比妥组能降低脑组 织缺血侧梗死面积比率、提高大鼠的空间学习记忆能力、防止 KCC2 蛋白表达过度下降。结 论： 苯巴比妥对新生大鼠缺氧缺血性脑损伤有保护作用，其机制可能与其防止 KCC2 蛋白 表达下降有关。 关键词：分子生物学；脑保护；缺氧缺血；苯巴比妥；KCC2 中图分类号：R966（分子药理学） Effect of Phenobarbital on the neonatal rats with hypoxic-ischemic brain damage and KCC2 level in the brain WANG Cong1, ZHANG Supei1, MA junyuan1, LI Qiang2,3, LI Jie2,3 (1. School of Pharmaceutical Sciences,Zhengzhou University, ZhengZhou 450001; 2. The First Affiliated Hospital of Henan College of Traditional Chinese Medicine, ZhengZhou 450000; 3. Key Laboratory of Viral Diseases Prevention and Treatment of Traditional Chinese Medicine of Henan Province, ZhengZhou 450000) Abstract: Objective: To explore the possible protective effect mechanisms of Phenobarbital, the present study was designed to observe its effect on the neonatal rats with hypoxic-ischemic brain damage and KCC2 level in the brain. Methods: Seven-day-old Sprague-Dawley (SD) rat pups were randomly divided into: (l) sham operation group (2) HIBD group (3) Phenobarbital treatment group. Rat HIBD model was prepared according the Rice-Vannucci method by ligating the left common carotid artery，followed by exposure to 8% oxygen and 92% nitrogen for 2 hours in the HIBD group and the Phenobarbital treatment group. At 24h after hypoxia–ischemia (HI), the pups were decapitated and brain morphological injury was assessed by 2, 3, 5-triphenyltetrazolium chloride (TTC), At 3 weeks after HI, Morris water maze test were conducted. In addition, the expression of potassium