雌性和雄性虎纹捕鸟蛛粗毒蛋白质含量比较分析.docVIP

  雌性和雄性虎纹捕鸟蛛粗毒蛋白质含量比 较分析# 王美迟，段志贵，胡朝暾，刘珍* 5 10 15 20 25 30 35 40 （湖南师范大学生命科学学院，蛋白质化学及发育生物学教育部重点实验室，长沙 410081） 摘要：自然界虎纹捕鸟蛛雌蛛数量远多于雄蛛数量，用不同方法比较了单个虎纹捕鸟蛛雌、 雄蛛粗毒的特征。结果表明雌蛛单次螫毒量为 26.5±2.47μl，冻干后粗毒质量为 5.01± 0.78mg，明显高于雄蛛单次螫毒量 10.83±1.35μl，冻干后粗毒质量为 2.05±0.17mg；用 Lowry 法和 Bradford 法测定雌蛛和雄蛛粗毒的蛋白质含量，两种方法均表明等量粗毒中雄蛛蛋白 总含量比雌蛛高。用反相高效液相色谱分离雌蛛和雄蛛粗毒蛋白，215nm 紫外扫描结果表明 大部分主峰重叠，雌蛛峰面积大，而雄蛛色谱图中多两个主峰。Tricine SDS电泳结 果表明雌蛛粗毒分子量小于 10kDa 的蛋白质含量偏高；而 Tris SDS电泳结果表明雌 蛛粗毒分子量大于 10kDa 的蛋白质含量偏低，这也许是雌蛛总蛋白含量偏低的主要原因。 研究结果提示了对雌、雄蛛粗毒独立研究的重要性。 关键词：蛋白质；虎纹捕鸟蛛；雌蛛；雄蛛 中图分类号：Q512 Female and male Ornithoctonus huwena crude venom protein content comparison analysis Wang Meichi, Duan Zhigui, Hu Zhaotun, Liu Zhen (Key laboratory of protein chemistry and developmental biology of the ministry of education, the college of life science, Hunan normal university, Changsha 410081) Abstract: The number of female spiders Selenocosmia huwena is greater than that of male spiders in nature, and we study the different characteristics of their venom using different methods. The results show that female single sting venom quantity is 26.5 ± 2.47 μ L, is significantly higher than that of males,which is 10.83 ± 1.35 μ L; freeze-dried crude venom quality of females was 5.01 ± 0.78mg, the male spiders is 2.05 ± 0.17mg; Using Lowry and Bradford methods to test the protein content, the results show that protein content of male spider was greater than that of female spider. We also seperate the female and male spider venom using reversed phase high performance liquid chromatography at 215nm UV scanning, the results show that most of the peaks are overlapped, two peaks are unique in male’s chromatogram. The results of tricine SDSelectrophoresis show that protein content of the female spider venom is more than that of male spider when molecular weight of proteins are less than 10kDa; it is converse in tris SDSelectrophoresis when molecular weight of proteins are more than 10kDa. Results suggest that the crude toxin of males should be st