柑橘黄龙病兼性厌氧型伴生细菌的分离及优势菌群分析.pdfVIP

植物病理学报 ACTAPHYTOPATHOLOGICA SINICA 41(5)：546—550(2011) 柑橘黄龙病兼性厌氧型伴生细菌的 分离及优势菌群分析 李颜方 ，殷幼平 ，王玉玺 ，李 佳 ，陈世伟。，王中康 (重庆大学生物工程学院基因工程研究中心，重庆 400030；农业部全 国农 技术推广服务中心，北京 100126 。』’。利添生物科技发展 (合浦)有限公司，北海 536128) Isolationoffacultativeanaerobicand entophyticbacteriacompanionedHuanglong— bing pathogen—infected citrus tissues and determination of dominant bacterial populations LIYan—fang ，YIN You—ping，WANG Yu．xi。，LIJia，CHEN Shi—wei。，WANG Zhong—kang ‘ (Bioengineering College，ChongqingUniversity，Chongqing400030，China； Ministry ofAgriculture， AgriculturalTechniqueExtensionandServiceCenter，Beijing100126，China；LuckyTeam BiotechDevelopment(Hepu) Ltd．，C0．Hepu，Beihai536128，China) Abstract：ToanalyzethediversityofentophyticbacteriaandfindthecompanionmicrobeinHuanglongbing pathogen—infectedandhealthycitrusplanttissues，variouspartsofciturstissueswerecollectedfrom different locationsofcitursplantingareas．Thefacultativeanaerobicandentophyticbacteriawereisolatedandidentified basedonbacterialmorphology，physiology，biochemistrycharacteristicsandPCR—DGGEanalysis(denaturing gradientgelelectrophoresis，analysisofthesequenceof16SrRNAV6一V8fragment)．Bythedirectionalisola— donofthefacultativeanaerobicandentophyticbacteriaand 16SrDNA amplificationmethods，total12genera ofbacteriawere identified from 19 cultivablebacterialpopulations．Thedominantbacterialpopulationwere Bacillussp．(IF：21．03％)，Planococcussp．(IF：20．69％)andPseudomonassp．(IF：17．44％)inhealhty citrustissues，whileCurtobacterium sp．(IF：29．07％)，Bacillussp．(IF：23．12％)，Microbacteriurnsp． (IF：21．09％)ininfectedcitursplants．BasedontheresultsofDGGE，9generaofculturedbacteriawere identified．ThedominantbacteriapopulationwasSerratiasp．(IF：28％)，andPantoeasp．(IF：14％)was secondonlytoit． ‘CandidatusLiberibacterasiaticus’wasfoundonly in tangerinepithofdeformed orange furit，whichsuggestedthatthecontents(l％)ofHuanglongbingweremoreindiseasedfurits