石蒜属不同种间生物碱含量差异性研究[J].pdfVIP

江西农业大学学报 2010, 32 3 : 0560 - 0565 http: / /xuebao. jxau. edu. cn Acta Agriculturae Universitatis J iangxiensis E - mail: ndxb7775@ sina. com 石蒜属不同种间生物碱含量差异性研究 1 2 3 袁菊红 ,胡绵好 ,夏 冰 1.江西财经大学 园林系 ,江西 南昌 330032; 2.江西财经大学 资环系 ,江西 南昌 330032; 3. 江苏省 ?中国科 学院 植物研究所 南京中山植物园 ,江苏 南京 210014 摘要 :利用高效液相色谱 HPLC 仪梯度洗脱程序对花谢后 2周的石蒜属植物和中国水仙 17份样品鳞茎中石 蒜碱、 力可拉敏和加兰他敏 3种生物碱含量进行测定 ,并对生物碱含量进行方差分析、 多重比较及相关分析 ,探 讨石蒜属生物碱含量的种间差异性及影响因素。结果表明 :所测 3种生物碱的含量在石蒜属种间存在很大的 差异 ,不同种间加兰他敏的含量为 0. 18~5. 15 mg/g,力可拉敏为 0. 20~2. 94 mg/g,石蒜碱为 1. 84~8. 36 mg/g。种 间差异达极显著水平 P 0. 01。同一物种石蒜碱与力可拉敏、 力可拉敏与加兰他敏含量呈一定的正相关 ,相 关系数分别为 0. 406 7和 0. 172 6,但石蒜碱与加兰他敏之间几乎没有相关性。被测石蒜属植物 3种生物碱含 量种间差异悬殊 ,同种不同产地间也存在差异 ,可作为石蒜属药用种质资源选择的依据。 关键词 :石蒜属 ;生物碱含量 ;种间差异 ; HPLC 中图分类号 : S682. 2 文献标志码 : A 文章编号 : 1000 - 2286 2010 03 - 0560 - 06 A Study on the D ifference in Alkaloids Contents in D ifferent Species of L ycoris 1 2 3 YUAN Ju2hong , HU M ian2hao , X I A B ing 1. Department of Landscape A rchitecture, J iangxi University of Finance and Econom ics, Nanchang 330032, China; 2. Resource and EnvironmentDepartment of J iangxiUniversity of Finance and Econom ics, Nan2 chang 330032, China; 3. Institute of Botany, J iangsu Province Chinese Academy of Sciences, Nanjing 210014, China Abstract: The contents of lycorine, lycoram ine, and galantham ine in bulbs of 17 samp les of L ycoris and N arcissus tazetta L. var. ch inensis were determ ined with HPLC technique, a variance analysis and multip le comparisons of the three alkaloids contents in different species were done, and the interspecific difference and influence factors of alkaloids contents were also discussed. The results indicated that there existed significant differences in three alkaloids contents in different L ycoris species P 0. 01The contents of galantham ine, lycoram ine and lycorine among of them were from 0. 18 to 5. 15, 0. 20 to 2. 9 4, and 1. 84 to 8. 36 mg/g re2 spectively. Correlation analysis showed that the relationship between the contents of lycorine and lycora