细胞膜片技术及其在骨组织领域的应用进展.pdfVIP

1122 Chinese Journal of Reparative and Reconstructive Surgery, September 2012, Vol. 26, No.9 · · 细胞膜片技术及其在骨组织领域的应用进展 陈亚莉 周诺 黄旋平 【摘  要】   目的 对细胞膜片技术（cell sheet technology，CST ）及其在骨组织领域的应用进展进行综述。  方 法 广泛查阅近年来国内外相关文献，并进行总结。 结果 CST 主要应用温度反应培养皿收获细胞，避免使用蛋白酶， 保留了培养过程中细胞自分泌的细胞外基质及相关蛋白和因子，将细胞以一层完整的膜状结构收集，为体内骨愈合提供了 适宜的微环境。以CST 为基础，通过同型或异型细胞膜片叠加、细胞膜片结合支架以及细胞膜片折叠等方法构建三维结 构组织，在组织工程骨构建方面表现出了巨大潜力。 结论 CST 与传统组织工程方法结合将促进骨组织工程学的发展。 【关键词】 骨组织工程 细胞膜片技术 温度反应培养皿 细胞外基质　 CELL SHEET TECHNOLOGY AND ITS APPLICATION IN BONE TISSUE ENGINEERING/CHEN Yali, ZHOU Nuo, HUANG Xuanping. Department of Oral and Maxillofacial Surgery, the Affiliated Hospital of Stomatology, Guangxi Medical University, Nanning Guangxi, 530021, P.R.China. Corresponding author: ZHOU Nuo, E-mail: nuozhou@ 【Abstract 】 Objective To review the progress of cell sheet technology (CST) and its appl ication in bone tissue engineering. Methods The l iterature concerning CST and its appl ication was extensively reviewed and analyzed. Results CST using temperature-responsive culture dishes is appl ied to avoid the shortcomings of traditional tissue engineering. All cultured cells are harvested as intact sheets along with their deposited extracellular matrix. Avoiding the use of proteolytic enzymes, cell sheet composed of the cells and extracellular matrix derived from the cells, and remained the relative protein and biological activity factors. Consequently, cell sheet can provide a suitable microenvironment for the bone regeneration in vivo. With CST, cell sheet engineering is allowed for tissue regeneration by the creation of three-dimensional structures via the layering of individual cell sheets, be created by wrapping scaffold with cell sheets, or be created by folding t