蜡样芽孢杆菌D-11降解粉末壳聚糖制备水溶性低聚糖研究.pdfVIP

现代食品科技 Modern Food Science and Technology 2012, Vol.28, No.9 蜡样芽孢杆菌D-11降解粉末壳聚糖 制备水溶性低聚糖的研究 1 1 1 2 3 1 高星爱 ,张永锋 ,赵新颖 ,刘思言 ,朴鲁东 ,金荣德 (1.吉林省农业科学院,吉林长春 1300212)(2.吉林农业大学生命科学学院,吉林长春 1301183) (3.韩国全南大学校应用生物工学部,韩国光州 500757) 摘要:为了省去制备胶状壳聚糖的工艺,能有效降解粉末壳聚糖利用到低聚糖的生产,利用自然环境中筛选得到的产壳聚糖酶 菌株蜡样芽孢杆菌 D-11,进行了基质条件优化实验。结果表明,以筛孔分别为 20、40、60、80目的粉末壳聚糖(3±0.5 cm;脱乙酰 度 98.1%)为基质,培养液含有 1%的吐温 60时,降解粉末壳聚糖的能力比对照提高 12%,当筛孔 60目的粉末壳聚糖为碳源(浓度 0.6%)时,降解粉末壳聚糖的能力提高 62.5%。随着基质每毫克中酶活性的提高,对粉末壳聚糖的分解率也随之增加。同时利用薄层 色谱法分析低聚糖的分布,有效证明了该 D-11菌株具有降解粉末壳聚糖的能力,且发现壳聚糖酶对基质粉末状态的降解率有选择性。 关键词:粉末壳聚糖;低聚糖;蜡样芽孢杆菌 D-11 文章篇号:1673-907820129-1149-1152 Preparation of Chito-oligosaccharides by Enzymatic Hydrolysis of Chitosan Powder 1 1 1 2 3 1 GAO Xing-ai , ZHANG Yong-feng , ZHAO Xin-ying , LIU Si-yan , PARK Rodong , JIN Rong-de 1.Jilin Academy of Agricultural Sciences, Changchun 130033, China 2.College of Life Sciences, Jilin Agricultural University, Changchun 130118, China 3.Division of Applied Bioscience and Biotechnology, Chonnam National University, Gwangju 500-757, Korea Abstract: In order to save the process of colloidal chitosan preparation in hydrolysis of chitosan powder to chito-oligosaccharide techniques. Chitosanse producing strains were isolated and identified as Bacillus cereus D-11. Twenty, forty, sixty and eighty meshes of powder chitosan 3±0.5 cm;D.D. 98.1% were used as carbon sources to investigate the optimal culture conditions of this strain. The chitooligosaccharides products were analyzed by Thin Layer Chromatography. Compared to control, the enzymatic activity of Bacillus cereus D-11 was increased by 12% and 62.5% in a medium containing 1% Tween 60 or 60 mesh powder chitosan, respectively. The yield of chito-oligosaccharides positively correlated with the enzyme amount. In conclusion, the product of chitosan which was hydrolyzed by chitosanse was determinded as chitooligosaccharides by TLC analysis. The hydrolysis rate of chitoasan by chitosanse was determinded by size of chitosanKey words