11株中度嗜盐菌的分离及生理生化特性研究.pdfVIP

安徽农业科学,Journal of Anhui Agri .Sci .2008 ,36(4) :1325 - 1326 , 1349 责任编辑 陈玉敏 责任校对 马君叶 11 株中度嗜盐菌的分离及生理生化特性研究 吕爱军, 胡秀彩, 张冬杰, 温洪宇 ( 徐州师范大学生命科学学院, 江苏徐州221116) 摘要 [ 目的] 了解海产品、盐渍类食品中的中度嗜盐菌资源, 探 究其生理 生化特性。[ 方法] 对海产品、盐渍品样 品进行分 离纯化, 获得 11 株 中度嗜盐菌, 对各菌株 的形态进行观察, 进行生理生化特征测试。[ 结果] 11 株 中度 嗜盐菌 中7 株为杆 菌, 其 中JD6 、HYD2 、NY80 和 NY11 革兰氏阳性, 其他革兰氏阴性,JD6 和JD910 产生芽孢;4 株为球菌, 其 中NY3 和 NY12 革兰氏阳性,XH1 和JH3 革兰氏阴性。11 株细 菌在0 ～20 % NaCl 条件下生长, 均分解葡萄糖产酸, 产生过氧化氢酶、赖 氨酸和 鸟氨酸脱羧酶, 不产生H S ; 菌株 XBD1 、XH1 、NY3 、JH3 和 2 NY12 在TCBS 与SS 琼脂上均能生长,NY11 和JD910 在SS 琼脂上生长。11 株细菌对氨苄青霉素和青霉素不敏 感, 对利福平敏 感 。11 株 细菌对小鼠无致死性。[ 结论] 该研 究为合理开发利用嗜盐微生物资源提供 了参考 。 关键词 中度嗜盐菌; 分 离; 生理生化特性 中图分类号 X 172 文献标识码 A 文章编号 0517 - 6611(2008) 04 - 01325 - 02 Studies on Isolation of 11Strains of Moderately Halophilic Bacteria and Their Physiological and Biochemical Characteristics LV Ai-j un et al ( College of Life Science , Xuzhou Nor mal University , Xuzhou , Jiangsu 221116) Abstract [ Obj ective] The research ai med to understand the resources of moderately halophilic bacteria in marine products and saline foods and probe into their physiological and biochemical characteristics . [ Method] The samples of marine products and saline foods were isolated and purified to obtai n 11 strains of moderately halophilic bacteria . The morphology of each strain was observed and their physiological and bioche mical characteristics were tested . [ Result] Among 11 strains of moderately halophilic bacteria , 7 strai ns were bacilli , in which JD6 , HYD2 , NY80 and NY11 were Gram positive , other ( XBD1 , JX181 and JD910) were Gramnegative and JD6 and JD910 generated spores . 4 strains were cocci , in which NY3 and NY12 were Grampositive , and XH1 and JH3 were Gram negative . 11 strains of bacteria could grow