肠腔灌注高氧液对缺血-再灌注后肠黏膜屏障损伤的保护作用.pdfVIP

686 20088 24 8 J Clin Anesthesiol, August 2008, Vol. 24, No. 8 高昌俊 柴伟 孙绪德 张贵和 赵晖 杨永慧 蒯建科 于代华 徐礼鲜 ! 24 , : ( I/ R ) (H OS ) (Sham ) Sham ( SMA) , SMA 1hH OS 20 ml kg- 1 h- 1 1 h, I/ R , 2 h , ATP ( ERO ) ; 2 ( ET) ; (T NF) ( Lac) ; Sham , I/ R , AT P ERO , 2 ET Lac T NF(P 005 P 0 01) , ; (H OS ) , ATP ERO ; ETLac TNF(P 005 P 001) , 2 (P 005) , ! ; ; ; Protective effects of intraluminal hyperoxygenated solution infusion on intestinal mucosal barrier injury induced by ischemiareperfusion GAO Changjun , CHAI Wei , SUN Xude, et al . Department of Anesthesiology, Tangdu Hosipit al , Fourt h Military Medical University, Xi∀ an 710038, China Abstract! Objective To investigate the protective effects of intraluminal hyperoxygenated solution ( H OS) infusion on intestinal mucosal barrier injury induced by ischemiareperfusion in rabbits.Methods Tw entyfour rabbits w ere random ly divided into three groups wit h 8 rats each. Sham operation w as performed in sham group. Intestinal ischem iareperfusion ( I/ R) model w as established by clamping superior mesenteric artery (SMA) w ith an atraumatic vascular clamp for 1h, and followed by reperfusion for 2 h.Animals in H OS group received intraluminal H OS infusion at the rate of 20 ml kg- 1 h- 1 for 1 h immediately after occlusion of SMA, and animals in I/ R group received the same volume of normal saline in the same way . After 2 h of reperfusion, the morphological changes of intestinal tissue w ere observed under optical microscope. Mucosal ATP cont ent and intest ine oxygen ext raction ratio ( ERO ) w ere determined. Cont ents of endotoxin in