大孔树脂分离纯化黑果枸杞总黄酮的研究.pdfVIP

※基础研究 食品科学 2009, Vol. 30, No. 01 19 李淑珍，李 进* ，杨志江，原 惠 (新疆师范大学生命科学与化学学院，新疆乌鲁木齐 830054) ：目的：研究大孔树脂分离纯化黑果枸杞总黄酮的工艺条件及参数。方法：利用单因素试验和多因素正 交试验研究大孔树脂分离纯化黑果枸杞总黄酮的最佳工艺条件。结果：AB-8 型大孔树脂分离纯化黑果枸杞总黄酮 的最佳工艺条件为：上样液浓度0.200mg/ml ，流速2ml/min ，pH5 ；以95% 的乙醇洗脱，流速4ml/min ，用量为 5 倍柱床体积。AB-8 树脂的饱和吸附量为0.64691mg/g 树脂，重复利用9 次吸附量仍然很好。采用该工艺分离纯化 黑果枸杞总黄酮含量达28% 。结论：AB-8 树脂分离纯化黑果枸杞总黄酮效果较好，工艺的重复性和稳定性良好， 适合于工业化生产。 ：黑果枸杞；总黄酮；大孔树脂；吸附纯化 Study on Separation and Purification of Total Flavonoids from Lycium ruthenicum Murr. with Macroreticular Resin LI Shu-zhen ，LI Jin* ，YANG Zhi-jiang ，YUAN Hui (College of Life Science and Chemistry, Xinjiang Normal University, Urumqi 830054, China) Abstract：Objective: To optimize process conditions of separation and purification of total flavonoids from Lycium ruthenicum Murr. with macroreticular resin. Methods: Siginal factor and orthogonal tests were done. Results: Using AB-8 resin to adsorb and purify total flavonoids from Lycium ruthenicum Murr, the optimal purification conditions are as follows: concentration of sample solution 0.200 mg/ml,velocity of sample solution 2 ml/min, pH 5, velocity of 95% ethanol taken as eluent 4 ml/min, and volume of 95% ethanol 5 BV (bed volume). The saturated adsorption capacity of AB-8 is 0.64691 mg/g resin, and after used 9 times repeatedly, adsorption capacity of AB-8 is still good. With these paramaters, the purity of product is 28.0%. Conclusion: AB-8 resin is effective in the adsorption and purification of the total flavonoids form Lycium ruthenicum Murr. with good repetition and stability. Therefore, the above technice is suitable to industrial production. Key words ：Lycium ruthenicum Murr. ；total flavonoids ；macroreticular resin ；