应用亲水作用液相色谱法测定食品中过氧化氢.pdfVIP

4 6 Vol. 4 No. 6 2013 12 Journal of Food Safety and Quality Dec. , 2013 1 2 1, 2* 1 孙 佳 , 毛 燕 , 李 刚 , 张 丽 (1. , 130118; 2. , 130022) 摘 要: 目的 方法 , , 1 mL/min , Venusil HILIC (250 mm×4.6 mm, 5 µm, 100 Å) , 结果 0.6 µg/mL, 2 µg/mL, R ≥30%, RSD≤7%结论 , 关键词: ; ; ; HILIC Application of hydrophilic interaction liquid chromatography to determine hydrogen peroxide in food 1 2 1, 2* 1 SUN Jia , MAO Yan , LI Gang , ZHANG Li (1. College of Food Science and Technology, Jilin Agricultural University , Changchun 130118, China; 2. Jilin Province Product Quality Supervision Test Institute , Changchun 130022, China) ABSTRACT: Objective To establish a high pressure liquid chromatography (HPLC) method for determination of the hydrogen peroxide content in food. Methods The food sample was directly extracted with water, and the residual hydrogen perioxide (H O ) was determined without derivatization via hydrophilic interaction 2 2 chromatography (HILIC) subsequently. The separation was carried out through a Venusil HILIC column (250 mm×4.6 mm, 5 µm, 100 Å) with acetonitrile as mobile phase at a flow rate of 1 mL/min. Results The detection limit and quantitation limit of H2O2 were 0.6 µg/mL and 2 µg/mL, respectively. The averag