衍生化法测定山药中还原型谷胱甘肽(GSH)和总巯基(-SH)含量.pdfVIP

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衍生化法测定山药中还原型谷胱甘肽(GSH)和总巯基(-SH)含量.pdf

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4 4 Vol. 4 No. 4 2013 8 Journal of Food Safety and Quality Aug. , 2013 (GSH) (-SH) 金京实, 沈丽, 崔胜云* (, 133002) 摘要: 目的 (GSH)(-SH)方法 4,4 ′- (4-DPS) (OPA), , UV-vis (-SH)GSH 结果 GSH, 0.4556 µmol/g 0.1792 µmol/g UV-vis 1.0458 µmol/L 0.5666 µmol/L, 98.73% 98.40% 结论关键词: ; ; 4,4′-; ; Determination of reduced glutathione (GSH) and total thiols (-SH) in Rhizoma Dioscoreae by derivatization methods * JIN Jing-Shi, SHEN Li, CUI Sheng-Yun (Key Laboratory of Natural Resources Functinal Molecules of Changbai Mountain, Ministry of Education, Yanbian University, Yanji 133002, China) ABSTRACT: Objective To detect reduced glutathione (GSH) and total thiols (-SH) in Rhizoma Dioscoreae by derivatization methods. Methods Total thiols and reduced glutathione in Rhizoma Dioscoreae were de- termined by UV-vis spectrophotometry and fluorophotometry using 4,4′-dithiodipyridine (4-DPS) and o-phthaldehyde (OPA) as derivatization agents. Samples were prepared by simultaneous derivatization during cell rupture, Results 0.4556 µmol/g of total thiols and 0.1792 µmol/g of GSH were detected in the sample . The detection limits for UV-vis spectrophotometry and fluorophotometry were 1.0458 µmol/L and 0.5666 µmol/L, and recoveries were 98.73% and 98.40%, respectively. Conclusion This study provides use