提高乙酰唑胺滴眼液眼内通透性的实验研究.pdfVIP

维普资讯 6l4 ChinOphthalRes．Dec{m1)er2004，Vo1．22．No．6 · 实 验 研 究 · 提高乙酰唑胺滴眼液眼内通透性的实验研究 陈 瑛 杨国华 陈祖基 徐 岩 张俊杰 Experimentalstudyonimprovingocularpenetrabilityofacetazolam ide ophthalmicsolution ChenYing，YangGuohua，ChenZuji，uYah，ZhangJunlie．HenanInstituteofOphthalmology， Zhengzhou450003，China Abstract Objective Toinvestigatethepromotingeffectofpenetrationenhancersonaeetazolamideeyedropinrabhit． M ethods Thirtyminutesafter instillation of50p．L ofaeetazolamide ophthahnie solution，the aqueoushumor sampleswere withdrawnand quantified by RP—HPLC， and the drug concentration in aqueous humorwas measured． Results The aeetazolamideconcentrationin aqueoushumorWaS (0．073 ±0．Ol3)，(0．250 ±0．075)and (0．232±0．074)I．tg／ml respectivelyat30 minutesaftertheinstillationofl％ ．3．3％ and 10％ aeetazolamidesolution．Comparedwiththecontrolgroup． 0．Ol％ benzalkonium bromide，1％ methykellul0se，l％ and5％ n—butylalcoholaswellas5％ ，10％ ，20％ dinlethylsulfoxide couldnotincreasetheconcentrationofacetazolamideinaqueoushumor(P0．05)．0．0l25％ 0．4％ ofazonesignifi(a·ntly enhancedtheconcentrationofaeetazolamideinaqueoushumorby5 ． 8—38．7times(P0．01)．respectively．Onthe()ther hand，theconcentrationsofazoneinophthahniesolutionshadapositivecorrelationwith theconeentrati0n )(facetaZOlanli(1ein aqueoushumor(r= 0．92．P0．01)． Conclusion A selectiveuseofpenetrationenhancerscau inlproveintraoeular penetrabilityofaeetazolamideeyedrops． Keywords acetazolamideophthahnicsolution；penetrationenhancer；ocularpenetrability；rabbit 摘要 目的 观察不同种类的穿透促进剂对乙酰唑胺滴眼液的眼 内通透性作用。 方法 每次给兔滴药50uL， 30nlin后抽取房水并进行反相高效液相色谱分析，计算房水中乙酰唑胺的质量浓度。 结果 l％、3．3％、l0％乙酰唑胺 滴眼液滴眼后30rain，前房药物质量浓度分别为 (0．073±0