烧灼大鼠表层巩膜静脉法诱导慢性青光眼模型的研究.pdfVIP

维普资讯 598 ChinOphthalRes，December2004，Vo1．22L ．6 · 实 验 研 究 · 烧灼大鼠表层巩膜静脉法诱导慢性青光眼模型的研究 蔺雪静 王诤华 王大博 杨文毅 Establishmentoftheratmodelofchronicglaucomabycauterizing episcleralvessels ManXuejing，WangJinghua，WangDabo，YangWenyi．DepartmentofOphthalmology，Affiliated HospitalofMedicalCollege，QingdaoUniversity，Qingdao266003，China Abstract Objective ToestablishachronicglaucomamodelinWistarrats． Methods Chronicglaucomamodelwas createdin21Wistarratsbycauterizingepiscleralvessels．Threeepiscleralvesselsofthelefteyeofeachratwerecauterized，and therighteyewasusedasconto1．Theintraocularpressure(1OP)inbothmodelandcontroleyeswasmeasuredandrecorded regularly，Eyesofexperimentalratwereenucleatedin2，4 and6monthsafteroperationtoobservethechangesofopticdiskand retina，andnumbersofretinalganglion cellsin centraland peripheralretinawere counted． Results The average IOP was (35．0±1．6)mmHginmodeleyeswithanapproximately1．8-foldmorethanthatofcontroleyes(19．4±1．1)mmHg，and elevatedIOPpersistedforatleast6monthsafteroperation．Noanycomplicationintheanteriorsegmentwasseeninmodeleyes． Retinalthicknessbecamethinnerwithanabout46％ lossofretinalganglioncellsintheperipheralretina，and36％ inthecentral retina．C／D ofmodeleyewasover0．7after6months Conclusion Establishmentofchronicglaucomamodelbyunilaterally cauterizingtheepiscleralvesselsinWistarratsoffersareproducibleandquantitativewayforglaucomatouspharmacologystudy， observationofdamageofopticnerveandneuroprotectjonaswellastreatmentoftheopticneuropathyassociatedwithglaucoma． Keywords glaucoma；animalmodel；rat；opticnerve 摘要 目的 利用大鼠建立慢性青光眼模型。 方法 选用成年Wistar大鼠，左眼为实验眼，右眼为对照眼，找到实 验眼的4条表层巩膜静脉，烧灼其中3条。定期测量和记录双眼眼压，观察眼前节情况；测量和比较双眼角膜水平直径 大小。分别于烧灼后2、4、6个月时摘除双侧眼球。观察大鼠视网膜及视盘的组织形态学改变、双眼视网膜厚度