HPLC法测定发芽糙米中γ-氨基丁酸含量.pdfVIP

208 2006, Vol. 27, No. 04 γ 1 1, 2 2 房克敏 ，李再贵 *，袁汉成 ，孟 颖 (1.中国农业大学食品科学与营养工程学院，北京 100083； 2.Waters中国有限公司北京实验室，北京 100027) ：建立一种发芽糙米中γ-氨基丁酸含量的高效液相色谱测定法。采用AQC(6-氨基喹啉基-N-羟基琥珀酰亚 氨基氨基甲酸酯)为柱前衍生试剂，色谱柱为Waters AccQ·Tag 氨基酸分析柱，梯度洗脱，紫外检测波长248.0nm。 γ-氨基丁酸的线性范围在0.10～1.0μmol/ml，线性关系良好，线性方程为Y=3.0×106X-7877.6，r=0.9999，回收 率为99.0%～100.5%。该方法易于操作、稳定、灵敏、准确。用该方法测定了16 种发芽糙米，结果显示其发 芽后的含量均显著高于未发芽糙米中的 γ-氨基丁酸含量。 ： γ- 氨基丁酸；AccQ·Tag 衍生；HPLC；发芽糙米 Determination of γ-Aminobutyric Acid in Germinated Brown Rice by HPLC 1 1, 2 2 FANG Ke-min ，LI Zai-gui *，YUAN Han-cheng ，MENG Ying (1.College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China； 2.Waters China Limited—Beijing Laboratory, Beijing 100027, China) Abstract ：A method for analysis of γ-aminobutyric acid(GABA) in germinated brown rice by high performance liquid chromatography(HPLC) was developed. A gradient elution was used for the separation and quantification of GABA pre-column derivatization with AQC. The column was Waters AccQ·Tag for free amino acids. GABA was determined with UV detector at 248.0nm. Good linearities were observed within the ranges from 0.10 to 1.0 with equation of Y=3.0×106X-7877.6 (r=0.9999). And the recoveries were 99.0%～100.5% for GABA. The method is simple, steady, sensitive, accurate. GABAs in sixteen types of germinated brown rice were determinated by the method. The results showed that the content of GABA in germinated brown rice is significantly higher than that in normal brown rice. Key words：