4种品牌压力蒸汽灭菌化学指示监测效果的比较.docVIP

压力蒸汽灭菌化学指示卡监测质量的有效性探讨 广东医学院第三附属医院 龚小玲 黄秋芳 【摘要】目的：监测不同品牌压力蒸汽灭菌化学指示卡进行灭菌质量判定的有效性，强调压力蒸汽灭菌监测中物理监测、化学监测、生物监测相结合的重要性。方法：选择四种不同品牌压力蒸汽灭菌化学指示卡，在确认脉动真空压力蒸汽灭菌器在正常灭菌程序,灭菌性能合格的前提下，模拟两次物理监测提示灭菌失效模式及一次正常灭菌状态进行化学指示卡监测与生物监测，并对监测结果进行比较分析。结果：实验一组在蒸汽压力小于0.2MPa，真空度0.07MPa，蒸汽未饱和，温度刚升至121℃即停止灭菌（0min），生物监测合格率为0，其中两种品牌压力蒸汽灭菌化学指示卡显示合格率为50%、70%；实验二组蒸汽压力0.2MPa，真空度0.07MPa，蒸汽未饱和，温度126℃即停止灭菌（0min），生物监测合格率为22.2%，其中两种品牌压力蒸汽灭菌化学指示卡显示合格率100%；对照组正常灭菌状态组，四种品牌化学指示卡显示全部达到灭菌合格标准，与生物监测结果一致。结论：不同品牌压力蒸汽灭菌化学指示卡在正常灭菌状态显示全部达到灭菌合格标准，与生物监测结果一致，但在温度、时间、蒸汽压力、蒸汽饱和度不达标状况下，生物监测合格率为0,出现压力蒸汽灭菌化学指示卡显示合格率高达50-100%，监测结果的有效性与生物监测结果存在显著性差异（P0.05）,有统计学意义。提醒操作人员必须全面掌握相关知识，严格遵守操作规程，不能仅看化学监测结果，需要更关注物理的参数(压力、温度、时间、蒸汽饱和度)，并且按要求每周每个灭菌器行生物监测一次，以免因为仅仅观察化学监测而造成最终结果合格的假象。 关键词：压力蒸汽灭菌化学指示卡 灭菌质量判定 有效性 [ Abstract ] Objective : Monitoring of different brands of pressure steam sterilization chemical indicator card effective sterilization quality judgment, emphasizes the importance of combining physical, chemical, biological monitoring monitoring monitoring pressure steam sterilization.Method: Choose four different brands of pressure steam sterilization chemical indicator card, in recognition of the pulsation vacuum pressure steam sterilizer in normal sterilization, sterilization performance under the premise of qualified, two times of simulation of physical monitoring prompt sterilization failure of chemical indicative cards monitoring and biological monitoring mode and a normal sterilization condition, and the monitoring results are compared.Results: The experimental group in the steam pressure is less than 0.2MPa, vacuum degree 0.07MPa, unsaturated steam temperature rose to 121 ℃, just stop sterilization ( 0min ), biological monitoring a pass rate of 0, of which two kinds of brand pressure steam sterilization chemical indicator shows the qualified rate is 50%, 70%; experimental group two 0.2MPa steam pressure, vacuum degree 0.07MPa, unsaturated vapor, temperature 126 ℃stop sterilization (0min ), biological monitoring a pass