热激胁迫可能通过降低脂肪与糖原的积累抑制涡虫的发育.docVIP

热激胁迫可能通过降低脂肪与糖原的积累抑制涡虫的发育 作者：刘培党,林兴凤,王大勇 【摘要】 目的:探讨热激胁迫对于涡虫发育以及脂肪和糖原积累的影响。方法:涡虫的发育通过体长与体质量来评价。分析35 ℃热激胁迫处理涡虫的体长与体质量变化以及实质细胞中脂肪与糖原积累情况,并评价热激胁迫处理涡虫中发育与脂肪或糖原积累之间可能的相关性。结果:与未经历热激处理的对照相比,热激胁迫处理不同长度时间(16、20、24、28与32 h)后涡虫体长与体质量显著减少或降低。与对照相比,热激胁迫处理导致涡虫实质细胞中的脂肪颗粒数量明显降低、尺寸显著变小。类似地,热激胁迫处理还导致涡虫实质细胞中的糖原积累的显著降低。进而,线性回归分析表明热激胁迫处理涡虫的体长与体质量均显著相关于实质细胞中脂肪颗粒的尺寸或糖原的含量。结论:热激胁迫处理可以明显抑制涡虫的发育,且热激胁迫处理涡虫中存在发育与脂肪或糖原积累之间的显著关联。 【关键词】 热激胁迫; 脂肪; 糖原; 发育; 涡虫 Freshwater freeliving planarians, an important component of the aquatic ecosystem, are distributed worldwide in unpolluted streams. Planarians have a high regenerative capacity, and every fragment is capable of growing into a complete individual if an individual planarian is cut into small fragments. Traditionally, planarians have been a favored model animal in developmental biology. They can be easily collected in large numbers, require only low culture and test medium volumes, and can be kept inexpensively in laboratory for the study of stem cell biology[1], neuroregeneration[2], and toxicology[3]. 　　So far, the characteristics of planarian make it a suitable organism for studying the effects of environmental stresses. The acute toxicities of eight widely used surfactants on oxidative stress and cholinesterase(ChE) activities were examined in planarian Dugesia japonica, and the differences in acute toxicity among these surfactants were at least in the range of three orders of magnitudes[3]. The catalase activity was significantly induced by copper exposure at concentrations of 40, 80, and 160 μg·L1, suggesting that the catalase levels in planarians could represent a biomarker for estimation of copper effects in freshwater ecosystem[4]. The effects of UVirradiation to two planarian species were also determined, and UVrays caused obvious alterations of mortality, behavior, and morphology in each species[5]. In addition, the number of neoblasts extremely increased in the area of damaged tissue, immediatel