参附注射液对大鼠短暂性局灶性脑缺血损伤的治疗时间窗.docVIP

参附注射液对大鼠短暂性局灶性脑缺血损伤的治疗时间窗 作者：郑 玉，熊利泽，朱正华，王 强，陈绍洋 【关键词】 参附注射液 关键词: 参附注射液;脑缺血;治疗时间窗 摘 要:目的 探讨参附注射液对局灶性脑缺血性损伤的治疗时间窗. 方法 80只雄性SD大鼠，随机分为8组（每组n=10）:对照组，即单纯缺血再灌注组;参附注射液治疗A～G组，分别于缺血再灌注后0，0.5，1.0，1.5，2.0，4.0和6.0h经腹腔注射参附注射液，剂量为10mL kg-1 .用右侧颈内动脉线栓法致大脑中动脉阻闭120min，拔出尼龙线即刻为再灌注时间.观察再灌注后24h时神经功能损害改变并评分，24h时处死动物，取大脑行TTC染色以测量脑梗死容积. 结果 24h时神经功能损害评分，A～F组分别为:0.5±0.3，0.5±0.4，0.6±0.5，0.8±0.4，0.8±0.3和0.9±0.3，明显低于对照组（2.1±0.8）及G组（1.5±0.7）（Plt;0.05）.脑梗死容积A～F组分别为（46.4±26.1），（60.2±25.8），（67.2±36.7），（76.0±39.7），（94.1±34.9）和（98.4±35.3）mm3 ;均明显小于对照组（182.6±39.6）mm3 及G组（139.1±42.8）mm 3 （Plt;0.05），A～F组间24h时神经功能损害评分和梗死容积无差别（Pgt;0.05）. 结论 参附注射液对局灶性脑缺血再灌注损伤的最佳治疗时机在缺血后4h以内. 　　Keywords:Shenfu solution;cerebral ischemia;therapeutic time window Abstract:AIM To investigate the therapeutic time window for Shenfu solution in transient focal cerebral ischemic injury in rats.METHODS 80male SD rats weighing280～350g were randomized into8groups（n=10in each group）:Con-trol group，receiving no pharmacological intervention，Groups A～G，receiving intraperitoneal injection of Shenfu solution（10mL kg-1 ）at0，0.5，1.0，1.5，2.0，4.0and6.0h respectively after120min middle cerebral artery occlu-sion（MCAO）with3-0nylon monofilament via the right in-ternal carotid artery.The neurological outcomes were evalu-ated24h after the reperfusion.The brain infarct volumes were then assessed by TTC staining.RESULTS The neuro-logical deficit scores（NDS）for Groups A～F24h after the reperfusion were0.5±0.3，0.5±0.4，0.6±0.5，0.8±0.4，0.8±0.3and0.9±0.3，respectively，which were obviously lower than those of the control group（2.1±0.8）and Group G（1.5±0.7）（Plt;0.05）.The infarct volumes of the control group（182.6±39.6）mm3 and Group G（139.1±42.8）mm3 were significantly larger than those of Groups A～F（46.4±26.1），（60.2±25.8），（67.2±36.7），（76.0±39.7），（94.1±34.9）and（98.4±35.3）mm3 ，respectively（Plt;0.05）24h after the reperfusion.There were no differences between Groups A～F in both NDS and infarct volume（