基质金属蛋白酶2及CD147在卵巢上皮性肿瘤中的表达.docVIP

基质金属蛋白酶2及CD147在卵巢上皮性肿瘤中的表达 【关键词】 基质金属蛋白酶-2 　　关键词: 基质金属蛋白酶-2;CD147;免疫组化;卵巢肿瘤 　　摘 要:目的 了解基质金属蛋白酶（MMP-2）及CD147在卵巢良、恶性上皮性肿瘤中的表达，探讨其与卵巢癌转移发生的关系. 方法 用免疫组化SP法对47例卵巢癌组织、19例交界性上皮性卵巢肿瘤、17例良性上皮性卵巢肿瘤组织中MMP-2及CD147的表达情况进行了检测. 结果 MMP-2在恶性及交界性上皮性卵巢肿瘤中的强阳性表达率分别为59%，42%，明显高于良性组强阳性表达率30%（Plt;0.05），在卵巢癌中MMP-2的表达与卵巢癌的临床分期有关，Ⅲ～Ⅳ期卵巢癌强阳性表达率71%，明显高于Ⅰ～Ⅱ期卵巢癌强阳性表达率37%（Plt;0.05），与分化程度无明显关联，但有淋巴结转移的病例强阳性表达率87%，明显高于无淋巴结转移组.CD147在恶性及交界卵巢上皮性肿瘤中的强阳性表达率分别为64%，57%，而良性组则未见CD147的表达.在卵巢癌中，CD147的强阳性表达率与临床分期有关，Ⅲ～Ⅳ期的癌组织强阳性表达率74%，明显高于Ⅰ～Ⅱ期强阳性表达率45%，在不同分化程度卵巢癌组织中CD147的强阳性率无明显差异，有无淋巴结转移的卵巢癌组织中CD147强阳性表达率差异显著. 结论 MMP-2及CD147均与卵巢癌的分期及淋巴结转移有关，CD147与MMP-2的产生关系密切. 　　Keywords:matrix metalloproteinase-2;CD147;immunohis-tochemistry;ovarian epithelial tumor 　　Abstract:AIM To investigate the expression of matrix met-alloproteinase-2（MMP-2）and CD147in ovarian epithelial　tumor.METHODS The expression of MMP-2and CD147was detected in malignant，borderline and benign ovarian tis-sues by immunohistochemical SP method.Monoclonal anti-bodies against MMP-2and CD147were used.RESULTS In-tense expression rates of MMP-2in the malignant，border-line，benign epithelial tumor tissues of ovary was59%，42%，30%respectively，There were a significant difference between malignant，borderline and benign tumors（Plt;0.05）.In ovarian cancer，the expression of MMP-2had a significant relevance to clinical phases and lymph node metas-tasis.The intense expression rate（71%）in phasesⅢ～Ⅳof ovarian cancer was much higher than that in phasesⅠ～Ⅱ（37%）;no relevance was observed with pathological grades.The intense expression rates of CD147in the malignant，bor-derline，benign ovarian epithelial tumor were64%，57%，0respectively.Significant difference was observed between ma-lignant，borderline and benign ovarian epithelial tumor.In o-varian cancer，the expression of CD147was associated with clinical phases;the intense expression rate（74%）in phasesⅢ～Ⅳof ovarian cancers was much higher than that in phas-esⅠ～Ⅱ（45%）.18of23cases with lymph node met