基质金属蛋白酶抑制基因RECK在早孕自然流产绒毛组织中的表达及意义.docVIP

基质金属蛋白酶抑制基因RECK在早孕自然流产绒毛组织中的表达及意义 作者：方媛媛，刘福民，朱学文，李晓博，万美容，刘小云，冯霞 【摘要】 目的 观察早期自然流产患者绒毛组织中RECK基因﹝基质金属蛋白酶(MMP)抑制基因﹞的表达及其与MMP-2、-9之间的关系。方法 采用半定量RT-PCR和Western blotting法检测20例早期自然流产患者和20例正常早期妊娠绒毛组织中RECK、MMP-2和MMP-9基因的表达情况。结果 ①早期自然流产患者绒毛组织中MMP-2及MMP-9的mRNA表达水平低于正常早期妊娠者，差异有统计学意义(Plt;0.05);RECK mRNA的表达高于正常早期妊娠者，差异有统计学意义(Plt;0.05)。②自然流产患者绒毛组织中MMP-2、MMP-9蛋白表达低于正常早期妊娠者，差异有统计学意义(Plt;0.05);RECK蛋白表达高于正常早期妊娠者，差异有统计学意义(Plt;0.05)。③RECK蛋白与MMP-2、MMP-9蛋白之间存在明显的负相关。结论 RECK表达的增加进而抑制MMP-2、MMP-9的活化，致使滋养细胞侵袭能力下降，可能与孕早期自然流产的发生有关。 【关键词】 自然流产，早期;绒毛;RECK;基质金属蛋白酶 　　Abstract： Objective To investigate the expression of matrix metalloproteinase suppressor gene RECK and its relationship with matrix metalloproteinase-9 (MMP-9) and MMP-2 in villi in patients with early spontaneous abortion (ESA). Methods The expression of RECK, mRNA and protein of matrix metalloproteinases MMP-9 and MMP-2 in villi of ESA patients (abortion group, n=20) and normal first trimester pregnant women (control group, n=20) was detected by semi-quantitative RT-PCR, Western blotting, respectively. The correlations between the expression of RECK and the expression of MMP-2, MMP-9 were analyzed by Pearson test. Results ①The expression of MMP-9 and MMP-2 mRNA was significantly lower in villi of the abortion group compared with that of the control group (Plt;0.05). The expression of RECK mRNA increased significantly in villi of the abortion group (Plt;0.05); ②Western blotting analysis demonstrated that the expression of MMP-9 and MMP-2 protein in villi of the abortion group was significantly lower than that of the control group (Plt;0.05). The expression of RECK protein in villi was significantly higher in the abortion group than in the normal control group (Plt;0.05); ③The expression of RECK protein in villi of first trimester had significant negative-correlation with that of MMP-2 and MMP-9.Conclusion The increase in the expression level of RECK inhibited the activation of MMP-9 and MMP-2, and resulted