小鼠正常肺细胞和肺癌细胞之间的力学特性比较研究.pdfVIP

网络出版时间：2014-03-03 12:16 网络出版地址：/kcms/doi/10.11844/cjcb.2014.03.0297.html 中国细胞生物学学报 Chinese Journal of Cell Biology 2014, 36(3): DOI: 10.11844/cj cb.2014.03.0297 小鼠正常肺细胞和肺癌细胞之间的力学特性比较研究 牛庆元 张晶晶 纪珍玲 李建亭 马德顺* (沈阳大学生命科学与工程学院, 沈阳110044) 摘要 该实验通过鬼笔环肽染色技术观察并比较了小鼠正常肺细胞和肺癌细胞的微丝差异, α 利用荧光抗体染色技术测定了小鼠单个正常肺细胞和肺癌细胞的 -SMA蛋白含量变化, 以及利用 CTFM法测定了小鼠正常肺细胞和肺癌细胞的牵引力变化。结果发现, 小鼠肺细胞癌变后, 细胞内 微丝骨架发生了变化, 影响了细胞的形态; α-SMA蛋白含量明显下降并且变得分散; 细胞投影面积 显著减少, 大约减少27%, 细胞牵引力也显著减小, 均方根值大约减少49%。这说明细胞骨架、细胞 α 的形态、 -SMA蛋白和细胞牵引力均与细胞的癌变过程密切相关。 关键词 小鼠肺细胞; 小鼠肺癌细胞; 细胞微丝; 细胞牵引力 Comparative Study on the Mechanical Characteristics of the Mice Lung Cells and Lewis Lung Carcinoma Niu Qingyuan, Zhang Jingjing, Ji Zhenling, Li Jianting, Ma Deshun* (College of Life Science and Engineering of Shenyang University, Shenyang 110044, China) Abstract In this article, we observed and compared the microfilament differences with phalloidin stain- ing technology, determined the α-SMA protein changes of single cell with fluorescent antibody staining technol- ogy, and researched the changes of the cell traction force with CTFM (Cell Traction Force Microscopy) method between the mice lung cells and lewis lung carcinoma. The results showed that the microfilament in lewis lung carcinoma changed, and the cell morphology was also affected. α-SMA protein was significantly decreased and dispersed; The cell projection area was significantly reduced by roughly 27%, and the cell tract