6周高强度跑台运动对大鼠关节液及滑膜白细胞介素-1β的影响①.pdfVIP

中国康复理论与实践2013 年5 月第19 卷第5 期 Chin J Rehabil Theory Pract, May. 2013, Vol. 19, No.5 - 448 - DOI : 10.3969/j.issn. 1006-9771.2013.05.013 ·基础研究· 6 周高强度跑台运动对大鼠关节液及滑膜白细胞介素-1β的影响① 1a 1a 1b 1a 2 赵景新 ，王瑜 ，何曼 ，金宇 ，白伦浩 [摘要] 目的 探讨高强度跑台运动对大鼠关节液及滑膜白细胞介素- 1β(IL- 1β) 的影响。方法 将24 只雄性Sprague-Dawley 大 鼠随机分为安静对照组和高强度运动组各12 只。高强度运动组进行6 周高强度跑台运动。取各组大鼠膝关节股骨内侧髁软骨及滑 膜组织，HE 染色观察其组织变化，免疫组化法检测IL- 1β在大鼠滑膜中的表达，酶联免疫吸附法(ELISA)检测大鼠关节液中IL- 1β 水平。结果 高强度运动组大鼠膝关节出现关节软骨损伤和滑膜炎表现，关节液中IL- 1β的表达高于安静对照组(P0.05) ，滑膜组 织中IL- 1β的表达明显高于安静对照组(P0.01) 。结论 高强度运动导致的关节软骨损伤、滑膜炎症，以及关节液、滑膜中IL- 1β的 异常表达可能是创伤性关节炎发生的机制之一。 [关键词] 运动；白细胞介素- 1β；骨关节炎；滑膜；滑液 Expression of Interleukin-1βin Synovia and Synovium of Rats after High Intensity Exercise ZHAO Jing-xin, WANG Yu, HE Man, et al. Department of Orthopedics, Affiliated Hospital of Chengde Medical College, Chengde 067000, Heibei, China Abstract: Objective To study the expression of interleukin- 1β (IL- 1β) in synovia and synovium of rats after high intensity exercise. Methods 24 healthy male Sprague-Dawley rats were randomly divided into control group and high intensity group, and the latter were forced to run 60 min at the speed of 28 m/min for 6 weeks. The histological changes of cartilage from the lesion areas of medial femoral con- dyle and synovium of knee joint were examined with HE stain and the expression of IL- 1βin synovium with immunohistochemistry, in sy- novial fluid with the enzyme-linked immunosorbent assay (ELISA). Results The articular cartilage of knee were injured and synovitis were found in high intensity group. The expression of IL- 1βboth in synovial fluid and in synovium of the knee were more in high intensity group than in control group (P0.05). Conclusion High intensity exercise could cause aticular cartilage injury, s