蛋白精氨酸甲基转移酶在E3大鼠哮喘模型中表达变化的研究.pdfVIP

窑716 窑 南方医科大学学报(JSouthMedUniv) 2010;30(4) 蛋白精氨酸甲基转移酶在E3大鼠哮喘模型中表达变化的研究 孙青竹袁焦芳芳袁杨旭东袁钟波袁蒋梅花袁李国良袁吕彬袁韩燕袁宁启兰袁张富军袁孙 健袁吕社民渊西安交通大学医 学院遗传与分子生物学系袁陕西 西安 710061冤 摘要院目的观察 1~6型蛋白精氨酸甲基转移酶渊PRMT 冤在E3 大鼠急性哮喘模型肺脏和脾脏中的表达变化遥 方法构 建卵清蛋白诱导的E3 大鼠急性哮喘模型袁分为对照组和哮喘组袁每组各 10只大鼠袁病理及生化检测评估模型构建情 况袁 半定量PCR 检测 1~6型PRMT 的表达差异遥 结果和对照组相比袁 哮喘组大鼠肺脏组织中PRMT1渊P0.01冤尧 PRMT2渊P0.01冤尧PRMT3渊P0.05冤尧PRMT5渊P0.05冤的表达有显著性升高袁而PRMT4渊P0.05冤的表达显著性降低曰哮喘 组大鼠脾脏组织中PRMT2渊P0.05冤尧PRMT5渊P0.05冤的表达也出现显著性升高遥 结论蛋白精氨酸甲基转移酶在E3 大 鼠急性哮喘模型肺脏和脾脏中有差异性表达袁这可能在哮喘发病的基因转录后调节过程中发挥着重要的作用遥 关键词院蛋白精氨酸甲基转移酶曰哮喘曰E3 大鼠 中图分类号院R394.3 文献标识码院A 文章编号院1673-4254(2010)04-0716-04 ExpressionofproteinarginineN-methyltransferasesinE3ratmodelsofacuteasthma SUNQing-zhu, JIAOFang-fang, YANGXu-dong, ZHONGBo, JIANGMei-hua, LIGuo-liang, L譈 Bin, HANYan, NINGQi-lan, ZHANGFu-jun,SUNJian,L譈She-min DepartmentofMedicalGeneticsandMolecularBiology,XianJiaotongUniversityCollegeofMedicine,Xian710061,China Abstract:ObjectiveToobservetheexpre ionofproteinarginineN-methyltransferase(PRMT)genesinthelungandspleen ofE3ratswithacuteasthma.MethodsE3ratswithovalbumin-inducedpulmonaryinflammationweredividedintotwogroup (n=10),andthevalidityoftheacuteasthmamodelwasevaluatedbyhistologicalobservationwithHEandPASstainingandby measurementofNOproduction.Semi-quantitativeRT-PCRwasemployedtodetecttheexpre ionsofPRMT1-PRMT6gene inthelungandspleenti uesoftherats. ResultsInthelungti ueoftheasthmaticrats, thegeneexpre ionsofPRMT1 (P0.01), PRMT2 (P0.01), PRMT3 (P0.05) and PRMT5 (P0.05) were significantlyincreased, but the expre ion of PRMT4gene (P0.05) wa ignificantlydecreasedascomparedwiththoseinthecontrolti ue. Inthespleenti ueofthe asthmaticrats, theexpre ionsofPRMT2(P0.05) andPRMT5genes(P0.05) showedasignificantincreaseascompared withthoseinthecontrolratti ue. ConclusionThegeneexpre ionsofPRMTsvarysignificantlybetweenasthmaticr