叶绿素铜钠盐联合环孢菌素A对免疫介导再生障碍性贫血模型小鼠的实验研究.DOCVIP

临床医学论文-叶绿素铜钠盐联合环孢菌素A对免疫介导再生障碍性贫血模型小鼠的实验研究 ??????????????????????? 作者：巨君芳 周平 张如富 魏克民 【摘要】? 　　目的 探讨叶绿素铜钠盐(SCC)联合环孢菌素A(CsA)治疗再生障碍性贫血(AA)的疗效。 方法 建立免疫介导AA模型小鼠，分别胃饲2种不同剂量SCC 12.5mg/(kg·d)+CsA 12.5mg/(kg·d), SCC 25mg/(kg·d)+CsA 12.5mg/(kg·d),以CsA，SCC［各25mg/(kg·d）］为阳性对照组,正常对照组及模型对照组胃饲生理盐水,每组灌胃0.5ml/d，分2次给药，连续14d，分别检测血象，另取股骨作骨髓病理切片。结果 SCC+CsA小剂量组WBC、HB、PLT均明显高于AA模型组，且HB，PLT分别高于SCC组和CsA组（P＜0.05）。骨髓病理切片显示SCC+CsA小剂量组骨髓增生较模型组小鼠(造血组织容量＜34%）改善明显(造血组织容量80%)。 结论 SCC+CsA促进AA模型小鼠造血功能，提高小鼠外周血白细胞、血红蛋白、血小板三系，对AA模型小鼠具有一定的治疗作用，且疗效好于单用SCC、CsA。 【关键词】? 再生障碍性贫血 叶绿素铜钠盐 环孢菌素A 实验研究 　　【Abstract】? Objective? To explore the therapeutic effect of Sodium Copper Chlorophyllin combined with Cyclosporin A in treating AA (Aplastic Anemia, AA) model mice . Methods? To establish immune-mediated AA model, BALB/c rats(equal number of male and female )were randomly divided into six groups: the normal group, the AA model group, the CsA group ,the SCC group, the combination of SCC and CSA group, the combination of SCC and CSA low-dose group. They were given distilled water, CsA and SCC ［Each 25 mg / (kg·d]］, SCC 12.5mg / (kg·d) + CsA 12.5mg / (kg·d), the SCC 25mg / (kg·d) + CsA 12.5mg / (kg·d) , 0.5 ml twice daily for 14 days respectively , then hemogram were tested and pathological section of femur bone marrow were observed by biopsy. Results? The number of WBC、HB、PLT of the AA model mice in the combination of? SCC and CsA low-does group was significantly higher than in the AA model group,the CsA group and the SCC group（P＜0.05), showing the combination of SCC and CsA low-dose group for WBC,HB,PLT?recovery was better than the single-used SCC and the CsA. Bone marrow biopsy showed bone marrow hyperplasia cf the SCC Combined With CSA low-dose group compared to the AA model rats (hematopoietic tissue capacity less than 34%) improved significantly (hematopoietic tissue capacity 80%). Conclusion?Sodium Copper Chlorophyllin combined with Cychosporin A has obviose effects on recovering the hematopoiesis of the AA mod