珠子参对S180荷瘤小鼠化疗的减毒作用.DOCVIP

临床医学论文-珠子参对S180荷瘤小鼠化疗的减毒作用 【摘要】? 　　目的：观察珠子参对S180荷瘤小鼠氟尿嘧啶（5fluorouracil，5FU）化疗的减毒作用。　　方法：30只昆明种小鼠右腋皮下注射肉瘤S180腹水瘤细胞建立荷瘤模型，随机分设模型组、5FU组和5FU+珠子参组。观察荷瘤小鼠治疗前后的一般情况；计数外周血中白细胞（white blood cell, WBC）、计算网织红细胞比值；记录小鼠生存时间。　　结果：5FU+珠子参组S180荷瘤小鼠外周血WBC及网织红细胞下降程度低于5FU组（P＜0.01）。5FU+珠子参组S180荷瘤小鼠平均存活时间为32.5 d，5FU组平均存活时间为23.5 d，两组比较差异有统计学意义（P＜0.01）。　　结论：珠子参可减轻5FU化疗后的骨髓抑制，并可延长荷瘤化疗小鼠生存时间。 【关键词】? 珠子参; 化学疗法; 肉瘤180; 小鼠 　　Rhizoma Panacis majori reduces toxicity of chemotherapy in S180bearing mice 　　Methods: S180 cells were subcutaneously injected to the right armpit of the mice to establish the S180bearing model, and thirty mice were randomly divided into untreated group, 5FU group and 5FU combined with RPM group (5FU plus RPM group). The general condition of S180bearing mice was observed. The white blood cells, red blood cells, and reticulocytes in the peripheral blood were counted, and the ratio of reticulocytes was measured too. The lifespan of the S180bearing mice was recorded. 　　Results: The white blood cell count and the ratio of reticulocytes in 5FU plus RPM group were higher than those in 5FU group (P0.01). The average lifespan of 5FU plus RPM group was 32.5 days and that of the 5FU group was 23.5 days; there was a significant difference in the lifespan between the two groups (P0.01). 　　Conclusion: RPM can reduce the toxic effect of 5FU and prolong the lifespan of S180bearing mice. 　　Keywords: Codonopsis convolvulacea; chemotherapy; sarcoma 180; mice 　　珠子参（Rhizoma Panacis majoris，RPM）又名扣子七，是五加科人参属植物珠子参的根茎，具有活血散瘀、补血止血和消肿止痛之功，主要分布于东喜马拉雅至中国西部山区。研究表明，珠子参水煎液可明显对抗环磷酰胺和60Co辐射所引起的骨髓造血功能损伤，使降低的红细胞（red blood cell，RBC）、血红蛋白（hemoglobin, Hb）和白细胞（white blood cell, WBC）明显升高，并可明显增加骨髓有核细胞及网织红细胞数［1］，但对恶性肿瘤使用化疗药物后引起的骨髓抑制和造血功能损伤有无减毒作用，尚无人报道。前期研究发现，珠子参水煎液有显著抑瘤作用（另文报道），本课题以S180荷瘤小鼠为实验对象，观察珠子参对化疗药物5氟尿嘧啶（5fluorouracil，5FU）毒副反应的作用。 　　1? 材料与方法 　　1.1? 实验材料 　　1.1.1? 实验动物与细胞株? 4～6周龄昆明近交系小鼠30只，雌雄各半，体质量16～18 g，动物合格证为医动字第19052号，由同济医科大学动物实验中心提供。小鼠肉瘤腹水细胞株S180，由华中科技大学同济医学院免疫学教研室沈关心教授惠