竹荪提取液对砷中毒大鼠血细胞参数的影响.DOCVIP

临床医学论文-竹荪提取液对砷中毒大鼠血细胞参数的影响 ?????????????????????作者：罗鹏，张爱华，吴应宽，汪希兰，潘雪莉 【摘要】? 目的： 分析竹荪提取液对砷中毒大鼠血细胞15项参数的影响，为防治砷中毒及竹荪资源的开发利用提供依据。方法： As2O3经口染毒，建立砷中毒动物模型，竹荪提取液灌胃14 d，采用F 820半自动血细胞分析仪测定实验动物血细胞参数。 结果： （1）与正常对照组比较，砷中毒模型组红细胞计数（RBC）、红细胞压积（HCT）、淋巴细胞绝对值（WSCC）、血小板（PLT）显著降低，P0.05或 P0.01；红细胞血红蛋白含量（MCH）、平均红细胞血红蛋白浓度（MCHC）显著升高，P0.05或P0.01；（2）与砷中毒模型组比较，竹荪治疗组RBC、血红蛋白（HGB）、HCT呈现升高趋势，MCH呈降低趋势，MCHC随竹荪剂量升高而降低，高剂量组差异具有显著性（P0.05），平均红细胞体积（MCV）、红细胞体积宽度（RDWCV）呈升高趋势且高于正常对照组；白细胞（WBC）、WSCC、中性粒细胞绝对值（WLCC）呈上升趋势；淋巴细胞百分数（WSCR）在竹荪低剂量组显著性降低（P0.05），而后随剂量增加呈上升趋势；中性粒细胞百分数（WLCR）在竹荪低剂量组显著性升高（P0.05），而后随剂量增加呈下降趋势；PLT持续降低，血小板分布宽度（PDW）、平均血小板体积（MPV）呈上升趋势，但差异无统计学意义。结论： AS2O3对实验大鼠血液系统有一定的损害作用，竹荪提取液对砷所致的大鼠血液系统红细胞、白细胞、血小板等指标具有一定的保护作用。 【关键词】? 砷中毒； 红细胞； 白细胞； 血小板； 竹荪提取液； 大鼠，Wister 　　［Abstract］ Objective: To analyze 15 blood parmeters in arseniasis rats that changed under the influence of extract from Dictyophora duplicate(EXD), so as to provide references for further study. Methods: Arsenic poisoning animal model was established by given AS2O3 to rats orally. EXT was than used to treat these rats orally for 14 days, and F820 semiautomatic blood corpuscle analyzer was adopted to analyze the blood parameters of experimental animals. Results: (1)Compared with those of normal control group (group C), RBC, HCT, WSCC, and PLT were lower and MCH, and MCHC were higher significantly in arsenic poisoning group (group AP) (P﹤0.05 or P﹤0.01); (2)In EXD treated groups (high and low doses), RBC, HGB, HCT,MCV, and RDWCV were higher than those of group AP, and MCV, and RDWCV were higher than those of group C. When dose increased, MCHC descended significantly in high dose group (P﹤0.05). WBC, WSCC, and WLCC increased more along with dose raise. WSCR descended significantly in the low dose group (P﹤0.05), but increased more when dose increased. WLCR had opposite display to WSCR. PLT continuously reduced along with dose raise. PDW and MPV showed a rise trend, but it was not statistically significant. Conclusions: AS2O3