补肾生血药促进大鼠缺血后肢血管新生的实验研究.DOCVIP

临床医学论文-补肾生血药促进大鼠缺血后肢血管新生的实验研究 ?????????? 作者：梁天成 黄家应 向友 刘勇 苏松? 　【摘要】目的：探讨补肾生血药对缺血后肢大鼠外周循环中骨髓来源内皮祖细胞（EPC）的影响及缺血组织血管新生的影响。方法：建立大鼠后肢缺血模型，喂食补肾生血药，1周后细胞培养观察外周血EPC数量变化，4周后检测缺血组织微血管密度及血管内皮生长因子（VEGF）的表达情况。结果：实验组大鼠外周血培养的EPC（89.1±10.3/mm2）明显高于对照组（48.6±7.5/mm2)(P＜0.01)，缺血组织微血管密度（288.76±12.54）和VEGF表达（0.233±0.014）均高于对照组（224.84±5.87）,(0.162±0.018,P＜0.05)，且外周血EPC数量与微血管密度之间具有显著相关性（P＜0.01）。结论：补肾生血药通过动员骨髓EPC的迁移、分化、促进局部的血管新生，达到改善供血目的。 ??? 【关键词】植物药疗法?缺血?四肢?内皮祖细胞?新生血管化，生理性?血管?内皮生长因子 ????? Effect of Bushenshengxue traditiond medicine on angiogenesis of ischemic hindlimbs in rats ??? 【ABSTRACT】Objective:To explore the effect of limb ischemia on mobilization of bone marrow derivedendothelial pregenitor cells(EPCs) and neovascularization of ischemic tissue with traditional medicine.Methods:Unilateral ischemic model of the hindlimbs was produced by excision of the common and superficial femoral artery in 20 male rats.The other 20 animals served as the control group.At 1 week after operation the changing of the circulating EPCs was observed by mononuclear cells culture.Microvesseldensity,the VEGF expression and angiograph of ischemic limbs was detected at week 4.Results:The number of circulating EPCs in experiment group (89.1±10.3/mm2 VS 48.6±7.5/mm2,P＜0.01).Microvessel density of ischemic limbs was significantly higher than that in control group (288.76±12.54/mm2 VS 224.86±5.87/mm2,P＜0.01).The VEGF expression in experiment group was significantly higer than that in control group (A:0.233±0.014 VS 0.162±0.0186,P＜0.01).The number of circulating EPCs was greatly related to the microvessel density and VEGF expression (P＜0.01).Conclusion:Local perfusion of ischemic limb can be augmented by improving neovascularization with EPCs mobilization of bushenshengxue traditional medicine. ??? 【KEY WORDS】Phytotherapy?Extremities?Endothelial progenitor cells?Neovascularization,physiologic?Blood vessels?Endothelial growth factors??? 内皮祖细胞（endothelial progenitor cell ,EPC）是一种能定向分化为血管内皮细胞的前体细胞，主要存在于骨髓内，可参与机体在特