肿瘤坏死因子α功能抑制剂对肺气肿大鼠气道炎症的影响.DOCVIP

临床医学论文-肿瘤坏死因子α功能抑制剂对肺气肿大鼠气道炎症的影响 ????????????????????? 作者：李军利, 陈平, 张程, 张湘燕 【摘要】? 目的： 探讨肿瘤坏死因子α（TNFα）抑制剂——重组人Ⅱ型肿瘤坏死因子受体-抗体融合蛋白（rhTNFR:Fc）对肺气肿大鼠气道炎症的影响。方法: 48只大鼠分为正常对照组、肺气肿组、rhTNFR:Fc干预组（干预组）和干预对照组，单纯吸烟法建立肺气肿模型；后3组烟雾暴露达1月时，干预组给予rhTNFR:Fc进行干预，干预对照组给予空白对照制剂；熏烟80 d后，处理所有大鼠，正常对照组和肺气肿组肺组织切片行苏木素-伊红染色观察形态学改变，定量测定肺平均内衬间隔（MLI）、平均肺泡数（MAN）；支气管肺泡灌洗液（BALF）计数各组白细胞总数、中性粒细胞和巨噬细胞计数及其百分比，用酶联免疫吸附法（ELISA）测定各组血清及BALF中的TNFα浓度。结果: (1)肺气肿组MLI高于正常对照组，MAN低于正常对照组（P＜0.05）；(2)肺气肿组血清TNFα浓度高于正常对照组和rhTNFR:Fc干预组（P＜0.05），和干预对照组比较差异无显著性（P＞0.05）；肺气肿组BALF中TNFα浓度高于正常对照组（P＜0.05），但和rhTNFR:Fc干预组、干预对照组比较差异无统计学意义（P＞0.05）；(3)肺气肿组BALF中白细胞总数、中性粒细胞计数及百分比高于正常对照组（P＜0.01，P＜0.05和P＜0.05）和rhTNFR:Fc干预组（P＜0.01，P＜0.05和P＜0.05），与干预对照组比较差异无统计学意义（P＞0.05）。结论: TNFα与吸烟大鼠的肺气肿形成有关，并影响其气道炎症；rhTNFR:Fc对减轻其气道炎症具有一定作用。 【关键词】? 重组人融合蛋白质类； 肺气肿； 肿瘤坏死因子α； 气道炎症 　　［Abstract］Objective: To investigate the effects of tumor necrosis factorα (TNFα) inhibitor and recombinant human tumor necrosis factorFc (rhTNFR:Fc) on airway inflammation of emphysema rat models. Methods: Fortyeight rats were randomly divided into 4 groups: normal control group (group N),? emphysema group (group E),? rhTNFR:Fcinterfered group (group R) and interfering control group (group I). Emphysema rat models (rats in groups E, R, and I) were established by exposure to cigarette smoking daily for 1 month. Rats in group R were then treated with rhTNFR:Fc and those in group I were injected with certain praeparatum instead. After being exposed to cigarette smoking for 80 days, all of the animals were sacrificed and lung tissue sections were made and stained by hematoxylin and eosin（HE）, upon which morphological changes were observed, mean linear intercept (MLI）and mean alveolar number (MAN) were determined. The levels of TNFα in serum and bronchoalveolar lavage fluid (BALF) were detected with ELISA. Total cell counts, neutrophil counts and neutrophil proportion in BALF were examined. Results: MLI was higher and MAN was lower in group E than those in group N. Serum TNF