非小细胞肺癌组织中血小板反应素的表达和血管生成的关系.DOCVIP

临床医学论文-非小细胞肺癌组织中血小板反应素的表达和血管生成的关系 ??????????? 作者：董济民，呼晓荣，孙埃雄，何积银，李秀霞? 【关键词】? 凝血酶敏感 ??? Thrombospondin1, microvessel density in primary nonsmall cell lung cancer and their clinical relevance 　　【Abstract】AIM：? To explore the relationship between the expression of thrombospondin (TSP) and angiogenesis in nonsmall cell lung cancer (NSCLC). METHODS: By using SP immunohistochemistry stainning method, expression of TSP1 and TSP2 and microvessel counting were evaluated in surgically resected specimens from 55 patients with NSCLC and 30 adjacent normal tissues using the antiTSP1, antiTSP2 and antiCD34 monoclonal antibodies, respectively. RESULTS:?Active angiogenesis was observed in NSCLC tumor tissues. The microvessel density (MVD) was related to metastasis and the clinical stage of NSCLC. TSP1, TSP2 expressed mainly in the cytoplasm, nuclear and extracellular matrix. The expression of TSP1 and TSP2 in the 55 tumor tissues was significantly higher than that in the 30 adjacent normal tissues（158.70 ± 211 versus 14120±7.20 and 149.10±2.94 versus 145.70±［yn1］4.74, respectively, P0.05）. The expression of TSP1 and TSP2 in NSCLC patients was related to the clinical stage and metastasis of NSCLC. There was a negative correlation between TSP1, TSP2 expression and MVD (r＝-0.964,-0.876, respectively, P0.05). TSP1 played a more important role. CONCLUSION:? It indicates that TSP1 and TSP2 are closely correlated to the angiogenesis of NSCLC. TSP1 and TSP2, together with MVD, may serve as clinical predictors for the metastasis of NSCLC. 　　【Keywords】? carcinoma, nonsmall cell lung; thrombospondins; microvessel density; immunohistochemistry; image analyze gray value 　　【摘要】目的： 探讨血小板反应素（TSP）的表达及微血管密度（MVD）改变与非小细胞肺癌(NSCLC)的关系.方法： 用SP免疫组织化学技术，检测了55例NSCLC癌组织和30例肺癌旁组织中TSP1和TSP2的表达及MVD的改变.结果： NSCLC癌组织中存在活跃的血管生成；MVD与NSCLC侵袭转移和临床分期有关.肺癌组织中TSP1和TSP2阳性表达的图像分析灰度值（1587±21和1491±29）明显高于30例肺癌旁组织阳性表达的图像分析灰度值（1412±72和1457±47）（P005）； TSP1和TSP2阳性表达与NSCLC患者的病情进展和转移明显有关.TSP1和TSP2表达与MVD成负相关(r＝-0964，-0876,P001)