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临床医学论文-韩氏针刺改善海洛因依赖大鼠学习记忆障碍和海马NOS、AchE的关系
【摘要】? 目的 初步探讨韩氏针刺对海洛因依赖大鼠学习记忆的影响及其作用机制。方法 按逐日递增的原则皮下注射海洛因建立成瘾模型,利用Morris水迷宫测定大鼠成瘾后及针刺治疗后学习记忆的变化,用蛋白定量考马斯亮兰法测定海马组织中一氧化氮合酶(NOS)和乙酰胆碱酯酶(AchE)活性。结果 ①Morris水迷宫定位航行实验中,成瘾组逃避潜伏期较对照组明显延长(P0.05),而针刺组较成瘾组明显缩短(P0.05);空间探索实验中,成瘾组在第4象限搜索时间及120 s内跨越安全岛的次数较对照组明显下降(P0.05),而针刺组较成瘾组明显增加(P0.05)。②成瘾组海马组织中NOS和AchE活性高于对照组(P0.05),针刺组明显低于成瘾组(P0.05),而对照组和针刺组相比无明显差异。结论 韩氏针刺可以改善海洛因依赖大鼠学习记忆障碍,降低海洛因依赖引起的海马内NOS和AchE活性的增高。
【关键词】? 海洛因依赖;一氧化氮合酶;乙酰胆碱酯酶;学习记忆;针刺;大鼠
??? Abstract: Objective? To make a preliminary discussion of the effect of Han′s acupuncture stimulation on improving the learning and memory disorder of heroin-dependent rats and investigate its potential mechanisms. Methods? Animal model of drug addiction was established by subcutaneous injection of heroin in rats twice a day for 9 days, according to the principle of increasing daily dose. The ability of special learning and memory was assessed with Morris Water Maze behavioral test for the changes from addiction to the improvement by acupuncture treatment. Total protein quantification kit (Coomassie brilliant blue) was used to detect the activity of nitric oxide synthase (NOS) and AchE in rat hippocampus. Results? ①Compared with the Control group, the mean escape latency in place navigation of the Model group was markedly prolonged (P0.05), while that in the Acupuncture group was shortened noticeably compared with the Model group (P0.05). In space probe test, the mean exploring time of the fourth quadrant and the times of crossing the island within 120 s of the Model group markedly decreased, compared with the Control group (P0.05), while those of the Acupuncture group markedly increased, compared with the Model group (P0.05). ②The activity of the NOS and AchE in rat hippocampus of the Model group was higher than the Control group (P0.05), while that of the Acupuncture group was lower than the Model group (P0.05), there was no obvious difference between the Control and the Acupuncture groups, tho
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