补肾益气法对全脑缺血灌注大鼠脑组织含水量、血清TNF—α、IL—1、IL—6含量的影响.pdfVIP

摘 要 本课压根据中医传统理论，结合现代相关研究，在导师郑绍周教授指导下，就 补肾益气法对全脑缺血再湘注大鼠脑含水f，血清肿启坏死因子 (TNF-a)，白细 胞介素1(IL-1)，白细胞介素6(IL-6)含t的影响进行了实验研究，并对补肾 益气法治疗缺血性中风的机理进行了理论研究 实验采用四动脉阻断造成全脑缺血再滋注模型，以复方仙灵脾注肘液代表补肾 ，将Wistar种成年大鼠随机分为复方仙灵脾高 (XH)、中(XM),低 (XL) 剂ti组，川芍嚓阳性对照组(Y).模型组(M)和假手术组U()。实脸结果表明:l.复 方仙灵脾注射液可明显降低全脑缺血30min和再滋注60min血清中TNF-a,IL-1, IL-6的含f，从而抑制脑缺血再滋注后免疫炎症性损伤，复方仙灵脾注射液高 (XH)、中(XI()剂量组作用优子川苟嚓注射液，而复方仙灵脾低剂ti组 (XL)作用 不及川芍嚓注射液.2复方仙灵脾注射液可明显降低脑组织含水2、拮抗脑水肿、 保护脑组织，其作用与川夸嗦注射液大体相当讨 本实脸研究证实，补肾益气法可降低脑缺血再洛注后TNF-。、IL一1,IL-6 等细胞因子的活性，调节2次脑损伤中的缺血性免疫炎症性损伤，拮抗脑水肿.改 普循环，保护神经细胞免受损害，从而保护缺血脑组织。 关健词:补肾益气v全脑缺血一再灌建 复方仙灵脾注射淤 大鼠 肿瘤坏死因子一a，白细胞介素一1 白细胞介紊-6 脑水肿、 实验研究 Abstract Inthissubject,wedetectedthebrainwatercontentandthelevelsoftumornecrosis factor-a(TNF-a)、interleukin-1(IL-1),interleukin-6(IL-6)intheserumofratswith thewholecerebralischemia-reperfusion,afterwetreatedthemwiththemethodof tonifyingthekidneyandinvigoratingqiaccordingtotraditionalchinesemedicineand modernresearch,withtheguidingofthepofessorZhengshaozhou. Thewholecerebralischemia-reperfusionratmodelwasmadebyusingthe occlusionoffourvessels.FuFangxianLingpi(FFXLP)injectionstandedforthemethod oftonifyingthekidneyandinvigoratingqi.Theratsweredividedrandomlyintomodel grorps(M),psrudo-operationgroups(J),HightgroupsofFFXLPinjection(XH),Middle groupsofFFXLPinjection(XM),LowergroupsofFFXLPinjection(XL)andchuan xiongQin(CXQ)groupsascontrol(Y).TheresultsshowedthatFFXLPinjectioncould decreaseobviouslythelevelsofTNF-a、IL-I、IL-6intheserumoftheratswiththe wholeceredralischemia-reperfusion.ThefuctionofXHandXMisbetterthanY,butthe functionofXLisnotgoodasY.TheresltsalsoshowedthatFFXLPinjectioncould decreaseobviouslythebrainwatercontent.ThefunctionofFFXLPinjectionisaboutas goodasCXQinjection Allabovemetionedsuggestedthatthemethodoftonifyingkidneyandinvigorating qicoulddecreasethelevelsofTNF-a、IL-1、IL-6inserumoftheratswithcerebral ischemia-reperf