银杏叶提取物对兔脊髓缺血再灌注损伤的保护作用.DOCVIP

临床医学论文-银杏叶提取物对兔脊髓缺血再灌注损伤的保护作用 ???????????? 作者：樊立宏，王坤正，程斌 【关键词】? 银杏叶提取物 ??? ［摘要］? 目的：探讨银杏叶提取物对兔脊髓缺血再灌注（ischemiareperfusion, IR）损伤的保护作用及其机制。方法：27只新西兰大白兔随机分为假手术组、模型组和银杏叶提取物治疗组，每组9只。建立兔脊髓IR模型。对脊髓IR前及IR后24、48 h实验兔后肢运动功能进行评分，检测缺血脊髓组织中超氧化物歧化酶（superoxide dismutase, SOD）和丙二醛（malondialdehyde, MDA）的含量，并检测缺血脊髓组织神经细胞的凋亡指数及Bcl2、Bax蛋白的表达水平。结果：银杏叶提取物可明显改善脊髓IR后实验兔的后肢运动功能；提高脊髓组织中SOD的活性，降低MDA的水平；通过上调Bcl2及下调Bax蛋白的表达，抑制IR所致的脊髓神经细胞的凋亡。结论：银杏叶提取物对脊髓IR损伤有一定的保护作用，其机制可能与减轻脊髓神经细胞脂质过氧化损伤和抑制神经细胞的凋亡有关。 　　［关键词］? 银杏叶提取物; 再灌注损伤; 细胞凋亡; 脊髓; 脂质过氧化作用 　　Protective effects of ginkgo leaf extracts on neurons in spinal cord after ischemiareperfusion injury in rabbits 　　ABSTRACT? Objective: To observe the protective effects of ginkgo leaf extracts on spinal cord after ischemiareperfusion (IR) injury in rabbits and to find out its possible mechanism. Methods: Twentyseven New Zealand white rabbits were randomly divided into three groups, which were shamoperation group, untreated group and ginkgo leaf extractstreated group. The locomotor scores of hindlimbs in rabbits after 24 and 48 h of reperfusion were evaluated, and the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in ischemia spinal cord were examined. The apoptotic index (AI) of neurons in spinal cord was detected by terminal deoxyribonucleotidyl transferase (TdT)mediated dUTPbiotin nick end labeling (TUNEL) method. The expressions of Bcl2 and Bax proteins were examined by streptavidinbiotin peroxidase method. Results: The locomotor scores of hindlimbs in rabbits after 24 and 48 h of reperfusion in the ginkgo leaf extractstreated group were obviously elevated as compared with those in the untreated group (P＜0.05). The activity of SOD was higher and the level of MDA was lower in ischemia spinal cord in the ginkgo leaf extractstreated group than those in the untreated group (P＜0.01). The decreased neuron AI and the expressions of upregulated Bcl2 protein and downregulated Bax protein were also observed in the ginkgo leaf ex