贝类中诺如病毒(norovirus)检测方法 普通RT-PCR方法和实时荧光RT-PCR检测英文意见稿.doc

贝类中诺如病毒(norovirus)检测方法 普通RT-PCR方法和实时荧光RT-PCR检测英文意见稿.doc

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Foreword This Standard was drafted according to GB/T 1.1-2009. This Standard replaces the SN/T 1635-2005 “Detection method of norovirus in shellfish-conventional RT-PCR and real-time RT-PCR”. This Standard differs from SN/T 1635-2005 as follows: ——The title has been revised. ——The name of the virus has been revised, norovirus substitute for Norwalk like viruses in this edition of Chinese version. Annex A of this standard is normative annex. This standard was proposed by Certification and Accreditation Administration of the People’s Republic of China(CNCA). This standard is under the jurisdiction of CNCA. This standard was drafted by Guangxi Entry-Exit Inspection and Quarantine Bureau of Peoples Republic of China and Shanghai Entry-Exit Inspection and Quarantine Bureau of Peoples Republic of China. Main drafters of this standard are Liu Junyi, Pan Liangwen, Zhang Shuya, Li Xiaohong, Wei Meiliang, Luo Zhaofei. This standard is a professional standard of entry-exit inspection and quarantine promulgated for the first time. Detection method of norovirus in shellfish -conventional RT-PCR and real-time RT-PCR Scope This standard specifies conventional RT-PCR and real-time RT-PCR method for the detection of norovirus in shellfish. This standard is applicable to detection of norovirus in shellfish. Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. GB/T 19495.2 Detection of genetically modified organism and derived products- General requirements for laboratories Terms and Definitions For the purposes of this standard, the following terms and definitions apply. Real-time fluorescence RT-PCR: Real-time fluorescence RT-PCR is set up on the basis of conventional RT-PCR by adding a specific probe. This probe is composed of a short (ca. 20-25 bases) oligod

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